000106657 001__ 106657
000106657 005__ 20230519145411.0
000106657 0247_ $$2doi$$a10.3390/plants10081527
000106657 0248_ $$2sideral$$a124653
000106657 037__ $$aART-2021-124653
000106657 041__ $$aeng
000106657 100__ $$aLanga Lomba, Natalia
000106657 245__ $$aActivity of anthracenediones and flavoring phenols in hydromethanolic extracts of Rubia tinctorum against grapevine phytopathogenic fungi
000106657 260__ $$c2021
000106657 5060_ $$aAccess copy available to the general public$$fUnrestricted
000106657 5203_ $$aIn this work, the chemical composition of Rubia tinctorum root hydromethanolic extract was analyzed by GC–MS, and over 50 constituents were identified. The main phytochemicals were alizarin-related anthraquinones and flavoring phenol compounds. The antifungal activity of this extract, alone and in combination with chitosan oligomers (COS) or with stevioside, was evaluated against the pathogenic taxa Diplodia seriata, Dothiorella viticola and Neofusicoccum parvum, responsible for the so-called Botryosphaeria dieback of grapevine. In vitro mycelial growth inhibition tests showed remarkable activity for the pure extract, with EC50 and EC90 values as low as 66 and 88 μg·mL−1, respectively. Nonetheless, enhanced activity was attained upon the formation of conjugate complexes with COS or with stevioside, with synergy factors of up to 5.4 and 3.3, respectively, resulting in EC50 and EC90 values as low as 22 and 56 μg·mL−1, respectively. The conjugate with the best performance (COS–R. tinctorum extract) was then assayed ex situ on autoclaved grapevine wood against D. seriata, confirming its antifungal behavior on this plant material. Finally, the same conjugate was evaluated in greenhouse assays on grafted grapevine plants artificially inoculated with the three aforementioned fungal species, resulting in a significant reduction in the infection rate in all cases. This natural antifungal compound represents a promising alternative for developing sustainable control methods against grapevine trunk diseases
000106657 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000106657 590__ $$a4.658$$b2021
000106657 592__ $$a0.765$$b2021
000106657 594__ $$a3.6$$b2021
000106657 591__ $$aPLANT SCIENCES$$b39 / 240 = 0.162$$c2021$$dQ1$$eT1
000106657 593__ $$aPlant Science$$c2021$$dQ1
000106657 593__ $$aEcology, Evolution, Behavior and Systematics$$c2021$$dQ1
000106657 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000106657 700__ $$aSánchez Hernández, Eva
000106657 700__ $$aBuzón Durán, Laura
000106657 700__ $$aGonzález García, Vicente
000106657 700__ $$0(orcid)0000-0003-3009-0935$$aCasanova Gascón, José$$uUniversidad de Zaragoza
000106657 700__ $$aMartín Gil, Jesús
000106657 700__ $$0(orcid)0000-0003-2713-2786$$aMartín Ramos, Pablo$$uUniversidad de Zaragoza
000106657 7102_ $$15011$$2500$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cArea Ingeniería Agroforestal
000106657 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000106657 773__ $$g10, 8 (2021), 1527 [17 pp.]$$tPlants$$x2223-7747
000106657 8564_ $$s1241338$$uhttps://zaguan.unizar.es/record/106657/files/texto_completo.pdf$$yVersión publicada
000106657 8564_ $$s2506656$$uhttps://zaguan.unizar.es/record/106657/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000106657 909CO $$ooai:zaguan.unizar.es:106657$$particulos$$pdriver
000106657 951__ $$a2023-05-18-13:55:17
000106657 980__ $$aARTICLE