Enzymatic synthesis of novel fructosylated compounds by Ffase from Schwanniomyces occidentalis in green solvents

Piedrabuena, David ; Rumbero, Ángel ; Pires, Elísabet (Universidad de Zaragoza) ; Leal-Duaso, Alejandro ; Civera, Concepción ; Fernández-Lobato, María ; Hernaiz, María J.

Resumen: The β-fructofuranosidase from the yeast Schwanniomyces occidentalis (Ffase) produces potential prebiotic fructooligosaccharides (FOS) by self-transfructosylation of sucrose, being one of the highest known producers of 6-kestose. The use of Green Solvents (GS) in biocatalysis has emerged as a sustainable alternative to conventional organic media for improving product yields and generating new molecules. In this work, the Ffase hydrolytic and transfructosylating activity was analysed using different GS, including biosolvents and ionic liquids. Among them, 11 were compatible for the net synthesis of FOS. Besides, two glycerol derivatives improved the yield of total FOS. Interestingly, polyols ethylene glycol and glycerol were found to be efficient alternative fructosyl-acceptors, both substantially decreasing the sucrose fructosylation. The main transfer product of the reaction with glycerol was a 62 g L−1 isomeric mixture of 1-O and 2-O-β-D-fructofuranosylglycerol, representing 95% of all chemicals generated by transfructosylation. Unexpectedly, the non-terminal 2-O fructo-conjugate was the major molecule catalysed during the process, while the 1-O isomer was the minor one. This fact made Ffase the first known enzyme from yeast showing this catalytic ability. Thus, novel fructosylated compounds with potential applications in food, cosmetics, and pharmaceutical fields have been obtained in this work, increasing the biotechnological interest of Ffase with innocuous GS.

Idioma: Inglés

DOI: 10.1039/D1RA01391B

Año: 2021

Publicado en: RSC Advances 11, 39 (2021), 24312-24319
ISSN: 2046-2069

Factor impacto JCR: 4.036 (2021)

Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 75 / 180 = 0.417 (2021) - Q2 - T2

Factor impacto CITESCORE: 5.9 - Chemical Engineering (Q2)

Factor impacto SCIMAGO: 0.667 - Chemistry (miscellaneous) (Q1) - Chemical Engineering (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA-ERDF/E37-20R
Financiación: info:eu-repo/grantAgreement/ES/MCIU/RTI2018-093431-B-100
Financiación: info:eu-repo/grantAgreement/ES/MCIU/PID2019-105838RB-C3-2
Financiación: info:eu-repo/grantAgreement/ES/MCIU/RTI2018-096037-B-I00

Tipo y forma: Article (Published version)

Área (Departamento): Área Química Orgánica (Dpto. Química Orgánica)