000108510 001__ 108510
000108510 005__ 20240104111817.0
000108510 0247_ $$2doi$$a10.3390/nano11102723
000108510 0248_ $$2sideral$$a125125
000108510 037__ $$aART-2021-125125
000108510 041__ $$aeng
000108510 100__ $$0(orcid)0000-0001-9802-8199$$aLópez-Royo, Tresa$$uUniversidad de Zaragoza
000108510 245__ $$aEncapsulation of Large-Size Plasmids in PLGA Nanoparticles for Gene Editing: Comparison of Three Different Synthesis Methods
000108510 260__ $$c2021
000108510 5060_ $$aAccess copy available to the general public$$fUnrestricted
000108510 5203_ $$aThe development of new gene-editing technologies has fostered the need for efficient and safe vectors capable of encapsulating large nucleic acids. In this work we evaluate the synthesis of large-size plasmid-loaded PLGA nanoparticles by double emulsion (considering batch ultrasound and microfluidics-assisted methodologies) and magnetic stirring-based nanoprecipitation synthesis methods. For this purpose, we characterized the nanoparticles and compared the results between the different synthesis processes in terms of encapsulation efficiency, morphology, particle size, polydispersity, zeta potential and structural integrity of loaded pDNA. Our results demonstrate particular sensibility of large pDNA for shear and mechanical stress degradation during double emulsion, the nanoprecipitation method being the only one that preserved plasmid integrity. However, plasmid-loaded PLGA nanoparticles synthesized by nanoprecipitation did not show cell expression in vitro, possibly due to the slow release profile observed in our experimental conditions. Strong electrostatic interactions between the large plasmid and the cationic PLGA used for this synthesis may underlie this release kinetics. Overall, none of the methods evaluated satisfied all the requirements for an efficient non-viral vector when applied to large-size plasmid encapsulation. Further optimization or alternative synthesis methods are thus in current need to adapt PLGA nanoparticles as delivery vectors for gene editing therapeutic technologies.
000108510 536__ $$9info:eu-repo/grantAgreement/ES/FIS-FEDER/PI17-00949$$9info:eu-repo/grantAgreement/EC/H2020/752349 /EU/Nanoparticle-based immunization, a novel therapeutic strategy for amyotrophic lateral sclerosis/NanoALS$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 752349-NanoALS$$9info:eu-repo/grantAgreement/ES/MCIU/RTI2018-099019-A-I00
000108510 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000108510 590__ $$a5.719$$b2021
000108510 592__ $$a0.839$$b2021
000108510 594__ $$a6.6$$b2021
000108510 591__ $$aPHYSICS, APPLIED$$b37 / 161 = 0.23$$c2021$$dQ1$$eT1
000108510 593__ $$aMaterials Science (miscellaneous)$$c2021$$dQ1
000108510 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b109 / 344 = 0.317$$c2021$$dQ2$$eT1
000108510 593__ $$aChemical Engineering (miscellaneous)$$c2021$$dQ1
000108510 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b55 / 179 = 0.307$$c2021$$dQ2$$eT1
000108510 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b53 / 108 = 0.491$$c2021$$dQ2$$eT2
000108510 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000108510 700__ $$0(orcid)0000-0002-6873-5244$$aSebastián, Víctor$$uUniversidad de Zaragoza
000108510 700__ $$0(orcid)0000-0002-7277-4318$$aMoreno Martínez, Laura$$uUniversidad de Zaragoza
000108510 700__ $$0(orcid)0000-0002-4678-7465$$aUson, Laura$$uUniversidad de Zaragoza
000108510 700__ $$0(orcid)0000-0002-1505-498X$$aYus, Cristina$$uUniversidad de Zaragoza
000108510 700__ $$0(orcid)0000-0002-9324-0446$$aAlejo, Teresa$$uUniversidad de Zaragoza
000108510 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, Pilar$$uUniversidad de Zaragoza
000108510 700__ $$0(orcid)0000-0001-5687-6704$$aOsta, Rosario$$uUniversidad de Zaragoza
000108510 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza
000108510 700__ $$0(orcid)0000-0002-7477-8742$$aManzano, Raquel$$uUniversidad de Zaragoza
000108510 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000108510 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000108510 7102_ $$11012$$2315$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Farmacología
000108510 773__ $$g11, 10 (2021), 2723 [23 pp.]$$pNanomaterials (Basel)$$tNanomaterials$$x2079-4991
000108510 8564_ $$s2870196$$uhttps://zaguan.unizar.es/record/108510/files/texto_completo.pdf$$yVersión publicada
000108510 8564_ $$s2731228$$uhttps://zaguan.unizar.es/record/108510/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000108510 909CO $$ooai:zaguan.unizar.es:108510$$particulos$$pdriver
000108510 951__ $$a2024-01-04-11:06:02
000108510 980__ $$aARTICLE