000109065 001__ 109065
000109065 005__ 20230519145444.0
000109065 0247_ $$2doi$$a10.1021/acsnano.0c03229
000109065 0248_ $$2sideral$$a122397
000109065 037__ $$aART-2021-122397
000109065 041__ $$aeng
000109065 100__ $$aHildebrand, S.
000109065 245__ $$aQuantification of Lipoprotein Uptake in Vivo Using Magnetic Particle Imaging and Spectroscopy
000109065 260__ $$c2021
000109065 5060_ $$aAccess copy available to the general public$$fUnrestricted
000109065 5203_ $$aLipids are a major source of energy for most tissues, and lipid uptake and storage is therefore crucial for energy homeostasis. So far, quantification of lipid uptake in vivo has primarily relied on radioactive isotope labeling, exposing human subjects or experimental animals to ionizing radiation. Here, we describe the quantification of in vivo uptake of chylomicrons, the primary carriers of dietary lipids, in metabolically active tissues using magnetic particle imaging (MPI) and magnetic particle spectroscopy (MPS). We show that loading artificial chylomicrons (ACM) with iron oxide nanoparticles (IONPs) enables rapid and highly sensitive post hoc detection of lipid uptake in situ using MPS. Importantly, by utilizing highly magnetic Zn-doped iron oxide nanoparticles (ZnMNPs), we generated ACM with MPI tracer properties superseding the current gold-standard, Resovist, enabling quantification of lipid uptake from whole-animal scans. We focused on brown adipose tissue (BAT), which dissipates heat and can consume a large part of nutrient lipids, as a model for tightly regulated and inducible lipid uptake. High BAT activity in humans correlates with leanness and improved cardiometabolic health. However, the lack of nonradioactive imaging techniques is an important hurdle for the development of BAT-centered therapies for metabolic diseases such as obesity and type 2 diabetes. Comparison of MPI measurements with iron quantification by inductively coupled plasma mass spectrometry revealed that MPI rivals the performance of this highly sensitive technique. Our results represent radioactivity-free quantification of lipid uptake in metabolically active tissues such as BAT.
000109065 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/E15-17R$$9info:eu-repo/grantAgreement/EC/H2020/657215/EU/Cell-surface immobilized vs. internalized magnetic nanoparticles for magnetic hyperthermia studies/OUTstandINg$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 657215-OUTstandINg$$9info:eu-repo/grantAgreement/ES/MINECO/BIO2017-84246-C2-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/RYC-2015-17640
000109065 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000109065 590__ $$a18.027$$b2021
000109065 592__ $$a4.611$$b2021
000109065 594__ $$a24.3$$b2021
000109065 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b13 / 180 = 0.072$$c2021$$dQ1$$eT1
000109065 593__ $$aEngineering (miscellaneous)$$c2021$$dQ1
000109065 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b20 / 345 = 0.058$$c2021$$dQ1$$eT1
000109065 593__ $$aPhysics and Astronomy (miscellaneous)$$c2021$$dQ1
000109065 591__ $$aCHEMISTRY, PHYSICAL$$b12 / 165 = 0.073$$c2021$$dQ1$$eT1
000109065 593__ $$aMaterials Science (miscellaneous)$$c2021$$dQ1
000109065 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b11 / 109 = 0.101$$c2021$$dQ1$$eT1
000109065 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000109065 700__ $$aLöwa, N.
000109065 700__ $$aPaysen, H.
000109065 700__ $$0(orcid)0000-0001-5559-8757$$aFratila, R.M.
000109065 700__ $$aReverte-Salisa, L.
000109065 700__ $$aTrakoolwilaiwan, T.
000109065 700__ $$aNiu, Z.
000109065 700__ $$aKasparis, G.
000109065 700__ $$aPreuss, S.F.
000109065 700__ $$aKosch, O.
000109065 700__ $$0(orcid)0000-0003-1081-8482$$aDe La Fuente, J.M.
000109065 700__ $$aThanh, N.T.K.
000109065 700__ $$aWiekhorst, F.
000109065 700__ $$aPfeifer, A.
000109065 773__ $$g15 (2021), 434-446$$pACS Nano$$tACS NANO$$x1936-0851
000109065 8564_ $$s715781$$uhttps://zaguan.unizar.es/record/109065/files/texto_completo.pdf$$yPostprint
000109065 8564_ $$s3131898$$uhttps://zaguan.unizar.es/record/109065/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000109065 909CO $$ooai:zaguan.unizar.es:109065$$particulos$$pdriver
000109065 951__ $$a2023-05-18-14:37:00
000109065 980__ $$aARTICLE