Enhanced Protein Crystallization on Nafion Membranes Modified by Low-Cost Surface Patterning Techniques

Polino, M. ; Portugal, C.A.M. ; Le The, H. ; Tiggelaar, R. ; Eijkel, J. ; Crespo, J.G. ; Coelhoso, I.M. ; Pina, M.P. (Universidad de Zaragoza) ; Mallada, R. (Universidad de Zaragoza)

Resumen: In this work, the influence of surface topography on protein crystallization over Nafion is investigated. Two types of Nafion-based membranes were modified by soft lithographic techniques in order to create different topographies at the micro and nano scale and subsequently tested. From an analysis of the induction time, nucleation, and crystal growth rate of trypsin from bovine pancreas, all of the patterned Nafion-based membranes show an enhanced nucleation and crystal growth. To provide additional insight into the experimental observations, the wettability properties of the prepared samples and the ratio of the Gibbs free energy of heterogeneous nucleation to homogeneous nucleation were evaluated. The crystallization outcome results from the combined effect of both the structural and chemical properties of the nucleant Nafion surface.

Idioma: Inglés

DOI: 10.1021/acs.cgd.9b00969

Año: 2020

Publicado en: CRYSTAL GROWTH & DESIGN 20, 4 (2020), 2174-2186
ISSN: 1528-7483

Factor impacto JCR: 4.076 (2020)

Categ. JCR: CRYSTALLOGRAPHY rank: 5 / 25 = 0.2 (2020) - Q1 - T1
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 67 / 178 = 0.376 (2020) - Q2 - T2
Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 128 / 333 = 0.384 (2020) - Q2 - T2

Factor impacto SCIMAGO: 0.965 - Chemistry (miscellaneous) (Q1) - Materials Science (miscellaneous) (Q1) - Condensed Matter Physics (Q1)

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)