Primary cilia presence and implications in bladder cancer progression and invasiveness

Iruzubieta, Pablo ; Castiella, Tomás ; Monleón, Eva (Universidad de Zaragoza) ; Berga, Carmen ; Muñoz, Guillermo (Universidad de Zaragoza) ; Junquera, Concepción. (Universidad de Zaragoza)

Resumen: Urothelial bladder cancer is the tenth most common cancer worldwide. It is divided into muscle and non-muscle invading bladder cancer. Primary cilia have been related to several cancer hallmarks such as proliferation, epithelial-to-mesenchymal transition (EMT) or tumoral progression mainly through signaling pathways as Hedgehog (Hh). In the present study, we used immunohistochemical and ultrastructural techniques in human tissues of healthy bladder, non-muscle-invasive bladder cancer (NMIBC) and muscle-invasive bladder cancer (MIBC) to study and clarify the activation of epithelial-to-mesenchymal transition and Hedgehog signaling pathway and the presence of primary cilia. Thus, we found a clear correlation between EMT and Hedgehog activation and bladder cancer stage and progression. Moreover, we identified the presence of primary cilia in these tissues. Interestingly, we found that in NMIBC, some ciliated cells cross the basement membrane and localized in lamina propria, near blood vessels. These results show a correlation between EMT beginning from urothelial basal cells and primary cilia assembly and suggest a potential implication of this structure in tumoral migration and invasiveness (likely in a Hh-dependent way). Hence, primary cilia may play a fundamental role in urothelial bladder cancer progression and suppose a potential therapeutic target.

Idioma: Inglés

DOI: 10.1007/s00418-021-01965-2

Año: 2021

Publicado en: HISTOCHEMISTRY AND CELL BIOLOGY 155 (2021), 547–560
ISSN: 0948-6143

Factor impacto JCR: 2.531 (2021)

Categ. JCR: MICROSCOPY rank: 4 / 9 = 0.444 (2021) - Q2 - T2
Categ. JCR: CELL BIOLOGY rank: 163 / 195 = 0.836 (2021) - Q4 - T3

Factor impacto CITESCORE: 7.3 - Biochemistry, Genetics and Molecular Biology (Q1) - Health Professions (Q1) - Medicine (Q1)

Factor impacto SCIMAGO: 0.944 - Histology (Q1) - Molecular Biology (Q1) - Medical Laboratory Technology (Q1)

Tipo y forma: Article (PostPrint)

Área (Departamento): Area Histología (Dpto. Anatom.Histolog.Humanas)
Área (Departamento): Área Anatomía Patológica (Dpto. Cirugía)