000110680 001__ 110680 000110680 005__ 20230519145558.0 000110680 0247_ $$2doi$$a10.1186/s12263-021-00689-1 000110680 0248_ $$2sideral$$a126792 000110680 037__ $$aART-2021-126792 000110680 041__ $$aeng 000110680 100__ $$aIacomino, G. 000110680 245__ $$aThe association of circulating miR-191 and miR-375 expression levels with markers of insulin resistance in overweight children: an exploratory analysis of the I.Family Study 000110680 260__ $$c2021 000110680 5060_ $$aAccess copy available to the general public$$fUnrestricted 000110680 5203_ $$aBackground: In recent years, the exciting emergence of circulating miRNAs as stable, reproducible, and consistent among individuals has opened a promising research opportunity for the detection of non-invasive biomarkers. A firm connection has been established between circulating miRNAs and glycaemic as well as metabolic homeostasis, showing that levels of specific miRNAs vary under different physio-pathological conditions. Objective: In this pilot study, we investigated the expression of candidate miRNAs, hsa-miR-191-3p and hsa-miR-375, in relation to biomarkers associated with insulin sensitivity in a subgroup (n=58) of subjects participating to the European I.Family Study, a project aimed to assess the determinants of eating behaviour in children and adolescents and related health outcomes. The sample included overweight/obese children/adolescents since overweight/obesity is a known risk factor for impaired glucose homeostasis and metabolic disorders. Biological targets of candidate miRNAs were also explored in silico. Results: We observed a significant association of the two miRNAs and early changes in glycaemic homeostasis, independent of covariates including country of origin, age, BMI z-score, puberty status, highest educational level of parents, total energy intake, energy from fats, energy from carbohydrates, and energy from proteins. Conclusion: Identification of circulating miRNAs associated with insulin impairment may offer novel approaches of assessing early variations in insulin sensitivity and provide evidence about the molecular mechanisms connected to early changes in glycaemic homeostasis. Trial registration: ISRCTN, ISRCTN62310987. Retrospectively registered, http://isrctn.com/ISRCTN62310987 © 2021, The Author(s). 000110680 536__ $$9info:eu-repo/grantAgreement/EC/FP7/266044/EU/Determinants of eating behaviour in European children, adolescents and their parents/I.FAMILY 000110680 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000110680 590__ $$a4.423$$b2021 000110680 592__ $$a0.967$$b2021 000110680 594__ $$a8.8$$b2021 000110680 591__ $$aNUTRITION & DIETETICS$$b44 / 90 = 0.489$$c2021$$dQ2$$eT2 000110680 593__ $$aGenetics$$c2021$$dQ2 000110680 591__ $$aGENETICS & HEREDITY$$b55 / 177 = 0.311$$c2021$$dQ2$$eT1 000110680 593__ $$aEndocrinology, Diabetes and Metabolism$$c2021$$dQ2 000110680 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000110680 700__ $$aLauria, F. 000110680 700__ $$aRusso, P. 000110680 700__ $$aVenezia, A. 000110680 700__ $$aIannaccone, N. 000110680 700__ $$aMarena, P. 000110680 700__ $$aAhrens, W. 000110680 700__ $$aDe Henauw, S. 000110680 700__ $$aMolnár, D. 000110680 700__ $$aEiben, G. 000110680 700__ $$aForaita, R. 000110680 700__ $$aHebestreit, A. 000110680 700__ $$aKourides, G. 000110680 700__ $$0(orcid)0000-0003-0454-653X$$aMoreno, L.A.$$uUniversidad de Zaragoza 000110680 700__ $$aVeidebaum, T. 000110680 700__ $$aSiani, A. 000110680 700__ $$aon behalf of the I.Family Consortium 000110680 7102_ $$11006$$2255$$aUniversidad de Zaragoza$$bDpto. Fisiatría y Enfermería$$cÁrea Enfermería 000110680 773__ $$g16, 1 (2021), 10 [9 pp]$$pGenes nutr.$$tGenes and Nutrition$$x1555-8932 000110680 8564_ $$s625546$$uhttps://zaguan.unizar.es/record/110680/files/texto_completo.pdf$$yVersión publicada 000110680 8564_ $$s2068424$$uhttps://zaguan.unizar.es/record/110680/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000110680 909CO $$ooai:zaguan.unizar.es:110680$$particulos$$pdriver 000110680 951__ $$a2023-05-18-15:57:34 000110680 980__ $$aARTICLE