Experimental study of the mechanical transmission of rabbit hemorrhagic disease virus (RHDV2/b) by Aedes Albopictus (Diptera: Clicidae) and Phlebotomus papatasi (diptera: psychodidae); 34447999

Calvete, C. ; Delacour, S. (Universidad de Zaragoza) ; Oropeza Velasquez, R. ; Estrada, R. (Universidad de Zaragoza) ; Sarto, M.P. ; Iguacel, L. ; Lucientes, J . (Universidad de Zaragoza) ; Calvo, J.H.

Resumen: Rabbit hemorrhagic disease (RHD) is caused by a lagovirus mainly affecting European rabbits (Oryctolagus cuniculus), although other European and North American lagomorph species are also susceptible to fatal infection by the new viral variant RHDV2/b. In the present work, direct mechanical transmission of the rabbit hemorrhagic disease virus (RHDV2/b variant) by the hematophagous Diptera Aedes albopictus (Skuse) (Diptera: Culicidae) and the sand fly Phlebotomus papatasi (Scopoli) (Diptera: Psychodidae) was tested. For each species, six and three laboratory rabbits were exposed to bites of dipterous females partially fed on RHDV2/b viral suspension 2 h and 24 h prior to exposure, respectively. The rabbits were then monitored for clinical changes and mortality for 35 d, and seroconversion was assessed by indirect ELISA. No rabbit died or showed clinical signs of disease, and seroconversion was recorded in two rabbits challenged with P. papatasi females fed the viral suspension 2 h prior to exposure. The number of RHDV2/b RNA copies/female was higher in Ae. albopictus than in P. papatasi but the decrease over time of RNA load in Ae. albopictus was greater than that in P. papatasi. The results of this study suggest the inability of Ae. albopictus to serve as a direct mechanical vector of RHDV2/b, but sand flies could play a role in the local transmission of RHD. © The Author(s) 2021. Published by Oxford University Press on behalf of Entomological Society of America.

Idioma: Inglés

DOI: 10.1093/jme/tjab148

Año: 2022

Publicado en: Journal of medical entomology 59, 1 (2022), 350-354
ISSN: 0022-2585

Factor impacto JCR: 2.1 (2022)

Categ. JCR: VETERINARY SCIENCES rank: 43 / 144 = 0.299 (2022) - Q2 - T1
Categ. JCR: ENTOMOLOGY rank: 30 / 100 = 0.3 (2022) - Q2 - T1

Factor impacto CITESCORE: 4.0 - Agricultural and Biological Sciences (Q2) - Immunology and Microbiology (Q3) - Veterinary (Q1) - Medicine (Q2)

Factor impacto SCIMAGO: 0.694 - Insect Science (Q1) - Veterinary (miscellaneous) (Q1) - Parasitology (Q2) - Infectious Diseases (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/A05-17R
Financiación: info:eu-repo/grantAgreement/ES/DGA/A14-20R
Financiación: info:eu-repo/grantAgreement/ES/INIA/E-RTA2014-00009-00-00

Tipo y forma: Article (Published version)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Proy. investigación HRA (Dpto. Patología Animal)