Variation of plasma levels of glucose and triglyceride in adult NMRI mice of both sexes with DL-ethionine-inducedfatty liver
López, A. A. ; Márquez, Y. C. ; Salas, Y. J. ; Plaza, M. A. (Universidad de Zaragoza) ; Murillo, M. D.
Resumen: El objetivo del presente estudio fue determinar las modificaciones plasmáticas de la glucosa (Gluc) y de los triglicéridos (TG) en el hígado graso no alcohólico (HGNA) inducido por DL-etionina en ratones IRMN (imagen por resonancia magnética nuclear) machos y hembras. Se distribuyeron en dos grupos de 10 animales de cada sexo: uno control y otro tratado con etionina (7, 5 mg/20 g peso corporal). Después de un ayuno de 48 horas, por venopunción coccígea se obtuvo una muestra de sangre con anticoagulante y en los plasmas se cuantificaron las concentraciones de Gluc y de TG mediante kit comercial. La eutanasia se practicó bajo ligera anestesia con éter. Se disecó el hígado, se evaluó su aspecto macroscópico y se obtuvo el homogeneizado hepático, en el que se determinó la concentración de TG. Una pequeña porción de hígado fijado en formol tamponado al 10% fue utilizado para su estudio histopatológico. El análisis estadístico se realizó mediante el programa SPSS versión 17.0 para Windows. Tanto en los machos como en las hembras, la administración de DL-etionina indujo metamorfosis grasa hepática con vacuolas lipídicas en el citoplasma de los hepatocitos, así como un aumento de la concentración de TG en el tejido hepático (p<0, 001). En las hembras se observó que el HG provoca un descenso de los niveles de TG plasmáticos (p<0, 01), resultado que está de acuerdo con la aumentada glicemia que presentaron (p<0, 05). Tales efectos se deberían a la resistencia a la insulina, anormalidad clave en el HGNA. En los machos no se modificó el nivel de TG circulantes, lo que respondería a la glicemia normal que mostraron. Como conclusión, la inducción de HG por acción de la DL-etionina afectó los parámetros plasmáticos estudiados en forma diferente en machos y hembras, quizás debido a variables adaptativas ante el HG, propias de cada sexo.
The objective of the present study was to determine the plasma modifications of glucose (Gluc) and triglycerides (TG) in non-alcoholic fatty liver (NAFL) induced by DL-ethionine in male and female mice NMRI (nuclear magnetic resonance imaging). They were distributed in two groups of 10 animals of each sex: one control and the other treated with ethionine (7.5 mg/20 g body weight). After fasting for 48 hours, a blood sample with anticoagulant was obtained by coccygeal venipuncture and the plasma concentrations of Gluc and TG were quantified using a commercial kit. Euthanasia was performed under light ether anesthesia. The liver was dissected, its macroscopic appearance was evaluated and the liver homogenate was obtained, in which the concentration of TG was determined. A small portion of liver fixed in 10% buffered formalin was used for histopathological study. Statistical analysis was carried out using SPSS software program version 17.0 for Windows. In both males and females, the administration of DL-ethionine induced hepatic fat metamorphosis with lipid vacuoles in the cytoplasm of hepatocytes, as well as an increase in the concentration of triglycerides in the liver tissue (p<0.001). In females, it was observed that NAFL causes a decrease in plasma TG levels (p<0.01), a result that is in agreement with the increased glycemia they presented (p<0.05). Such effects would be due to insulin resistance, a key abnormality in NAFL. In males, the level of circulating TG was not modified, which would respond to the normal glycemia they showed. In conclusion, the induction of NAFL by the action of DL-ethionine affected the plasma parameters studied differently in males and females, perhaps due to adaptive variables to NAFL, specific to each sex. © 2022 Universidad Nacional de Nordeste (UNNE). All rights reserved.
Idioma: Español
DOI: 10.30972/VET.3315874
Año: 2022
Publicado en: Revista veterinaria 31, 1 (2022), 23-28
ISSN: 1668-4834
Factor impacto CITESCORE: 0.2 - Veterinary (Q4) - Agricultural and Biological Sciences (Q4)
Factor impacto SCIMAGO: 0.139 - Veterinary (miscellaneous) (Q4) - Animal Science and Zoology (Q4)
Tipo y forma: Article (Published version)
Área (Departamento): Área Fisiología (Dpto. Farmac.Fisiol.y Med.L.F.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes.
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The objective of the present study was to determine the plasma modifications of glucose (Gluc) and triglycerides (TG) in non-alcoholic fatty liver (NAFL) induced by DL-ethionine in male and female mice NMRI (nuclear magnetic resonance imaging). They were distributed in two groups of 10 animals of each sex: one control and the other treated with ethionine (7.5 mg/20 g body weight). After fasting for 48 hours, a blood sample with anticoagulant was obtained by coccygeal venipuncture and the plasma concentrations of Gluc and TG were quantified using a commercial kit. Euthanasia was performed under light ether anesthesia. The liver was dissected, its macroscopic appearance was evaluated and the liver homogenate was obtained, in which the concentration of TG was determined. A small portion of liver fixed in 10% buffered formalin was used for histopathological study. Statistical analysis was carried out using SPSS software program version 17.0 for Windows. In both males and females, the administration of DL-ethionine induced hepatic fat metamorphosis with lipid vacuoles in the cytoplasm of hepatocytes, as well as an increase in the concentration of triglycerides in the liver tissue (p<0.001). In females, it was observed that NAFL causes a decrease in plasma TG levels (p<0.01), a result that is in agreement with the increased glycemia they presented (p<0.05). Such effects would be due to insulin resistance, a key abnormality in NAFL. In males, the level of circulating TG was not modified, which would respond to the normal glycemia they showed. In conclusion, the induction of NAFL by the action of DL-ethionine affected the plasma parameters studied differently in males and females, perhaps due to adaptive variables to NAFL, specific to each sex. © 2022 Universidad Nacional de Nordeste (UNNE). All rights reserved.
Idioma: Español
DOI: 10.30972/VET.3315874
Año: 2022
Publicado en: Revista veterinaria 31, 1 (2022), 23-28
ISSN: 1668-4834
Factor impacto CITESCORE: 0.2 - Veterinary (Q4) - Agricultural and Biological Sciences (Q4)
Factor impacto SCIMAGO: 0.139 - Veterinary (miscellaneous) (Q4) - Animal Science and Zoology (Q4)
Tipo y forma: Article (Published version)
Área (Departamento): Área Fisiología (Dpto. Farmac.Fisiol.y Med.L.F.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. Exportado de SIDERAL (2023-09-13-11:33:44)
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Record created 2022-06-17, last modified 2023-09-14