Cytokine profile and anti-inflammatory activity of a standardized conditioned medium obtained by coculture of monocytes and mesenchymal stromal cells (PRS CK STORM)

Lapuente, J. P. ; Blázquez-Martínez, A. ; Marco-Brualla, J. ; Gómez, G. ; Desportes, P. ; Sanz, J. ; Fernández, P. ; García-Gil, M. ; Bermejo, F. ; Martín, J. V. S. ; Algaba, A. ; De Gregorio, J. C. ; Lapuente, D. ; De Gregorio, A. ; Lapuente, B. ; de la Viñas Andrés, M. ; Anel, A. (Universidad de Zaragoza)

Resumen: Intercellular communication between monocytes/macrophages and cells involved in tissue regeneration, such as mesenchymal stromal cells (MSCs) and primary tissue cells, is essential for tissue regeneration and recovery of homeostasis. Typically, in the final phase of the inflammation-resolving process, this intercellular communication drives an anti-inflammatory immunomodulatory response. To obtain a safe and effective treatment to counteract the cytokine storm associated with a disproportionate immune response to severe infections, including that associated with COVID-19, by means of naturally balanced immunomodulation, our group has standardized the production under GMP-like conditions of a secretome by coculture of macrophages and MSCs. To characterize this proteome, we determined the expression of molecules related to cellular immune response and tissue regeneration, as well as its possible toxicity and anti-inflammatory potency. The results show a specific molecular pattern of interaction between the two cell types studied, with an anti-inflammatory and regenerative profile. In addition, the secretome is not toxic by itself on human PBMC or on THP-1 monocytes and prevents lipopolysaccharide (LPS)-induced growth effects on those cell types. Finally, PRS CK STORM prevents LPS-induced TNF-A and IL-1B secretion from PBMC and from THP-1 cells at the same level as hydrocortisone, demonstrating its anti-inflammatory potency. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Idioma: Inglés

DOI: 10.3390/biom12040534

Año: 2022

Publicado en: Biomolecules 12, 4 (2022), 534 [18 pp]
ISSN: 2218-273X

Factor impacto JCR: 5.5 (2022)

Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 70 / 285 = 0.246 (2022) - Q1 - T1

Factor impacto CITESCORE: 8.3 - Biochemistry, Genetics and Molecular Biology (Q1)

Factor impacto SCIMAGO: 1.074 - Biochemistry (Q1) - Molecular Biology (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B31-20R

Tipo y forma: Article (Published version)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)