000118853 001__ 118853
000118853 005__ 20240319081014.0
000118853 0247_ $$2doi$$a10.1186/s13071-022-05407-3
000118853 0248_ $$2sideral$$a130270
000118853 037__ $$aART-2022-130270
000118853 041__ $$aeng
000118853 100__ $$aPichler, Verena
000118853 245__ $$aGeographic distribution of the V1016G knockdown resistance mutation in Aedes albopictus: a warning bell for Europe
000118853 260__ $$c2022
000118853 5060_ $$aAccess copy available to the general public$$fUnrestricted
000118853 5203_ $$aBackground
Colonization of large part of Europe by the Asian tiger mosquito Aedes albopictus is causing autochthonous transmission of chikungunya and dengue exotic arboviruses. While pyrethroids are recommended only to reduce/limit transmission, they are widely implemented to reduce biting nuisance and to control agricultural pests, increasing the risk of insurgence of resistance mechanisms. Worryingly, pyrethroid resistance (with mortality < 70%) was recently reported in Ae. albopictus populations from Italy and Spain and associated with the V1016G point mutation in the voltage-sensitive sodium channel gene conferring knockdown resistance (kdr). Genotyping pyrethroid resistance-associated kdr mutations in field mosquito samples represents a powerful approach to detect early signs of resistance without the need for carrying out phenotypic bioassays which require availability of live mosquitoes, dedicated facilities and appropriate expertise.
Methods
Here we report results on the PCR-genotyping of the V1016G mutation in 2530 Ae. albopictus specimens from 69 sampling sites in 19 European countries.
Results
The mutation was identified in 12 sites from nine countries (with allele frequencies ranging from 1 to 8%), mostly distributed in two geographical clusters. The western cluster includes Mediterranean coastal sites from Italy, France and Malta as well as single sites from both Spain and Switzerland. The eastern cluster includes sites on both sides of the Black Sea in Bulgaria, Turkey and Georgia as well as one site from Romania. These results are consistent with genomic data showing high connectivity and close genetic relationship among West European populations and a major barrier to gene flow between West European and Balkan populations.
Conclusions
The results of this first effort to map kdr mutations in Ae. albopictus on a continental scale show a widespread presence of the V1016G allele in Europe, although at lower frequencies than those previously reported from Italy. This represents a wake-up call for mosquito surveillance programs in Europe to include PCR-genotyping of pyrethroid resistance alleles, as well as phenotypic resistance assessments, in their routine activities.
000118853 536__ $$9info:eu-repo/grantAgreement/EUR/COST-Action/CA17108
000118853 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000118853 590__ $$a3.2$$b2022
000118853 592__ $$a0.961$$b2022
000118853 591__ $$aTROPICAL MEDICINE$$b6 / 24 = 0.25$$c2022$$dQ1$$eT1
000118853 593__ $$aParasitology$$c2022$$dQ1
000118853 591__ $$aPARASITOLOGY$$b9 / 37 = 0.243$$c2022$$dQ1$$eT1
000118853 593__ $$aInfectious Diseases$$c2022$$dQ2
000118853 594__ $$a6.6$$b2022
000118853 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000118853 700__ $$aCaputo, Beniamino
000118853 700__ $$aValadas, Vera
000118853 700__ $$aMicocci, Martina
000118853 700__ $$aHorvath, Cintia
000118853 700__ $$aVirgillito, Chiara
000118853 700__ $$aAkiner, Mustafa
000118853 700__ $$aBalatsos, Georgios
000118853 700__ $$aBender, Christelle
000118853 700__ $$aBesnard, Gilles
000118853 700__ $$aBravo-Barriga, Daniel
000118853 700__ $$aBueno-Mari, Rubén
000118853 700__ $$aCollantes, Francisco
000118853 700__ $$0(orcid)0000-0001-7046-2997$$aDelacour-Estrella, Sarah$$uUniversidad de Zaragoza
000118853 700__ $$aDikolli, Enkelejda
000118853 700__ $$aFalcuta, Elena
000118853 700__ $$aFlacio, Eleonora
000118853 700__ $$aGarcía-Pérez, Ana L.
000118853 700__ $$aKalan, Katja
000118853 700__ $$aKavran, Mihaela
000118853 700__ $$aL’Ambert, Gregory
000118853 700__ $$aLia, Riccardo P.
000118853 700__ $$aMarabuto, Eduardo
000118853 700__ $$aMedialdea, Raquel
000118853 700__ $$aMelero-Alcibar, Rosario
000118853 700__ $$aMichaelakis, Antonios
000118853 700__ $$aMihalca, Andrei
000118853 700__ $$aMikov, Ognyan
000118853 700__ $$aMiranda, Miguel A.
000118853 700__ $$aMüller, Pie
000118853 700__ $$aOtranto, Domenico
000118853 700__ $$aPajovic, Igor
000118853 700__ $$aPetric, Dusan
000118853 700__ $$aRebelo, Maria Teresa
000118853 700__ $$aRobert, Vincent
000118853 700__ $$aRogozi, Elton
000118853 700__ $$aTello, Ana
000118853 700__ $$aZitko, Toni
000118853 700__ $$aSchaffner, Francis
000118853 700__ $$aPinto, Joao
000118853 700__ $$adella Torre, Alessandra
000118853 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000118853 773__ $$g15 (2022), 280 [7 pp.]$$pParasites & Vectors$$tParasites and Vectors$$x1756-3305
000118853 8564_ $$s1133368$$uhttps://zaguan.unizar.es/record/118853/files/texto_completo.pdf$$yVersión publicada
000118853 8564_ $$s2288816$$uhttps://zaguan.unizar.es/record/118853/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000118853 909CO $$ooai:zaguan.unizar.es:118853$$particulos$$pdriver
000118853 951__ $$a2024-03-18-15:29:31
000118853 980__ $$aARTICLE