| Página principal > Artículos > Borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells > MARC |
000119622 001__ 119622 000119622 005__ 20240319081026.0 000119622 0247_ $$2doi$$a10.1016/j.isci.2022.104793 000119622 0248_ $$2sideral$$a129771 000119622 037__ $$aART-2022-129771 000119622 041__ $$aeng 000119622 100__ $$0(orcid)0000-0003-4617-8130$$aAparicio Yuste, Raúl$$uUniversidad de Zaragoza 000119622 245__ $$aBorrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells 000119622 260__ $$c2022 000119622 5060_ $$aAccess copy available to the general public$$fUnrestricted 000119622 5203_ $$aBorrelia burgdorferi (Bb), a vector-borne bacterial pathogen and the causative agent of Lyme disease, can spread to distant tissues in the human host by traveling in and through monolayers of endothelial cells (ECs) lining the vasculature. To examine whether Bb alters the physical forces of ECs to promote its dissemination, we exposed ECs to Bb and observed a sharp and transient increase in EC traction and intercellular forces, followed by a prolonged decrease in EC motility and physical forces. All variables returned to baseline at 24 h after exposure. RNA sequencing analysis revealed an upregulation of innate immune signaling pathways during early but not late Bb exposure. Exposure of ECs to heat-inactivated Bb recapitulated only the early weakening of EC mechanotransduction. The differential responses to live versus heat-inactivated Bb indicate a tight interplay between innate immune signaling and physical forces in host ECs and suggest their active modulation by Bb. 000119622 536__ $$9info:eu-repo/grantAgreement/EC/H2020/101018587/EU/Individual and Collective Migration of the Immune Cellular System/ICoMICS$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101018587-ICoMICS$$9info:eu-repo/grantAgreement/ES/MCIU/FPU20-05274$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-124271OB-I00 000119622 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000119622 590__ $$a5.8$$b2022 000119622 592__ $$a1.624$$b2022 000119622 591__ $$aMULTIDISCIPLINARY SCIENCES$$b15 / 73 = 0.205$$c2022$$dQ1$$eT1 000119622 593__ $$aMultidisciplinary$$c2022$$dQ1 000119622 594__ $$a6.9$$b2022 000119622 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000119622 700__ $$aMuenkel, Marie 000119622 700__ $$aAxarlis, Konstantinos 000119622 700__ $$0(orcid)0000-0002-1878-8997$$aGómez Benito, María J.$$uUniversidad de Zaragoza 000119622 700__ $$aReuss, Annalena 000119622 700__ $$aBlacker, Grace 000119622 700__ $$aTal, Michal Caspi 000119622 700__ $$aKraiczy, Peter 000119622 700__ $$aBastounis, Effie E. 000119622 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est. 000119622 773__ $$g25, 8 (2022), 104793 [28 pp.]$$piScience$$tISCIENCE$$x2589-0042 000119622 8564_ $$s6750482$$uhttps://zaguan.unizar.es/record/119622/files/texto_completo.pdf$$yVersión publicada 000119622 8564_ $$s1687807$$uhttps://zaguan.unizar.es/record/119622/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000119622 909CO $$ooai:zaguan.unizar.es:119622$$particulos$$pdriver 000119622 951__ $$a2024-03-18-16:44:50 000119622 980__ $$aARTICLE
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