Alterations in bacterial metabolism contribute to the Lifespan Extension Exerted by Guarana in Caenorhabditis elegans

Reigada, Inés ; Kapp, Karmen ; Maynard, Claire ; Weinkove, David ; Valero, Marta Sofía (Universidad de Zaragoza) ; Langa, Elisa ; Hanski, Leena ; Gómez-Rincón, Carlota

Resumen: Guarana (Paullinia cupana) is a widely consumed nutraceutical with various health benefits supported by scientific evidence. However, its indirect health impacts through the gut microbi-ota have not been studied. Caenorhabditis elegans is a useful model to study both the direct and indirect effects of nutraceuticals, as the intimate association of the worm with the metabolites produced by Escherichia coli is a prototypic simplified model of our gut microbiota. We prepared an ethanoic extract of guarana seeds and assessed its antioxidant capacity in vitro, with a 2, 2-diphenyl-1-pic-rylhydrazyl (DPPH) assay, and in vivo, utilizing C. elegans. Additionally, we studied the impact of this extract on C. elegans lifespan, utilizing both viable and non-viable E. coli, and assessed the impact of guarana on E. coli folate production. The extract showed high antioxidant capacity, and it extended worm lifespan. However, the antioxidant and life-extending effects did not correlate in terms of the extract concentration. The extract-induced life extension was also less significant when utilizing dead E. coli, which may indicate that the effects of guarana on the worms work partly through modifications on E. coli metabolism. Following this observation, guarana was found to decrease E. coli folate production, revealing one possible route for its beneficial effects. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Idioma: Inglés

DOI: 10.3390/nu14091986

Año: 2022

Publicado en: Nutrients 14, 9 (2022), 1986 [15 pp.]
ISSN: 2072-6643

Factor impacto JCR: 5.9 (2022)

Categ. JCR: NUTRITION & DIETETICS rank: 17 / 87 = 0.195 (2022) - Q1 - T1

Factor impacto CITESCORE: 9.0 - Nursing (Q1) - Agricultural and Biological Sciences (Q1)

Factor impacto SCIMAGO: 1.291 - Nutrition and Dietetics (Q1) - Food Science (Q1)

Tipo y forma: Article (Published version)

Área (Departamento): Área Fisiología (Dpto. Farmac.Fisiol.y Med.L.F.)