000119841 001__ 119841
000119841 005__ 20240319081011.0
000119841 0247_ $$2doi$$a10.3389/fphar.2022.1009868
000119841 0248_ $$2sideral$$a130620
000119841 037__ $$aART-2022-130620
000119841 041__ $$aeng
000119841 100__ $$aCásedas, Guillermo
000119841 245__ $$aEvaluation of two different Cannabis sativa L. extracts as antioxidant and neuroprotective agents
000119841 260__ $$c2022
000119841 5060_ $$aAccess copy available to the general public$$fUnrestricted
000119841 5203_ $$aCannabis sativa L. is a plant that contains numerous chemically active compounds including cannabinoids such as trans-Δ-9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD), and flavone derivatives, such as luteolin-7-O-glucuronide and apigenin glucuronide. In particular, the polar fraction of hemp including many phenolic compounds has been overlooked when compared with the more lipophilic fraction containing cannabinoids. Therefore, the aim of this study was to assess two extracts of industrial hemp (C. sativa) of different polarity (aqueous and hexane) by evaluating their antioxidant profile and their neuroprotective potential on pharmacological targets in the central nervous system (CNS). Several assays on in vitro antioxidant capacity (DPPH, superoxide radical, FRAP, ORAC), as well as inhibition of physiological enzymes such as acetylcholinesterase (AChE) and monoaminooxidase A (MAO-A) were carried out in order to find out how these extracts may be helpful to prevent neurodegenerative disorders. Neuro-2a cell line was selected to test the cytotoxic and neuroprotective potential of these extracts. Both extracts showed striking antioxidant capacity in the FRAP and ORAC assays, particularly the hexane extract, and interesting results for the DPPH and superoxide radical uptake assays, with the aqueous extract standing out especially in the latter. In enzyme inhibition assays, the aqueous extract showed AChE and MAO-A inhibitory activity, while the hexane extract only reached IC50 value for AChE inhibitory bioassay. Neuro-2a assays demonstrated that polyphenolic extract was not cytotoxic and exhibited cytoprotective properties against hydrogen peroxide and antioxidant response decreasing reactive oxygen species (ROS) production. These extracts could be a source of compounds with potential benefit on human health, especially related to neurodegenerative disorders.
000119841 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000119841 590__ $$a5.6$$b2022
000119841 592__ $$a1.064$$b2022
000119841 591__ $$aPHARMACOLOGY & PHARMACY$$b44 / 278 = 0.158$$c2022$$dQ1$$eT1
000119841 593__ $$aPharmacology (medical)$$c2022$$dQ1
000119841 593__ $$aPharmacology$$c2022$$dQ1
000119841 594__ $$a6.3$$b2022
000119841 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000119841 700__ $$aMoliner, Cristina
000119841 700__ $$aMaggi, Filippo
000119841 700__ $$aMazzara, Eugenia
000119841 700__ $$0(orcid)0000-0001-6969-1055$$aLópez, Víctor
000119841 773__ $$g13 (2022), 1-11$$pFront. pharmacol.$$tFrontiers in Pharmacology$$x1663-9812
000119841 8564_ $$s1256249$$uhttps://zaguan.unizar.es/record/119841/files/texto_completo.pdf$$yVersión publicada
000119841 8564_ $$s1976591$$uhttps://zaguan.unizar.es/record/119841/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000119841 909CO $$ooai:zaguan.unizar.es:119841$$particulos$$pdriver
000119841 951__ $$a2024-03-18-15:10:15
000119841 980__ $$aARTICLE