000119974 001__ 119974
000119974 005__ 20240319081025.0
000119974 0247_ $$2doi$$a10.3390/ijms231911882
000119974 0248_ $$2sideral$$a130379
000119974 037__ $$aART-2022-130379
000119974 041__ $$aeng
000119974 100__ $$aSánchez-Hernández, Eva
000119974 245__ $$aHolm oak (quercus ilex subsp. ballota (desf.) samp.) bark aqueous ammonia extract for the control of invasive forest pathogens
000119974 260__ $$c2022
000119974 5060_ $$aAccess copy available to the general public$$fUnrestricted
000119974 5203_ $$aHolm oak (Quercus ilex subsp. ballota (Desf.) Samp.) bark is a commonly used remedy to treat gastrointestinal disorders, throat and skin infections, hemorrhages, and dysentery. It has also been previously reported that its methanol extracts possess antibacterial activity, which can be related to the richness of Quercus spp. extracts in phenolic compounds, such as flavonoids and tannins. However, there is no information on the antifungal (including oomycete) properties of the bark from Q. ilex or its subspecies (ilex and ballota). In this work, we report the characterization of the aqueous ammonia extract of its bark by FTIR and GC-MS and the results of in vitro and ex situ inhibition tests against three phytopathogens. The main phytochemical components identified were inositols (19.5%), trans-squalene (13%), 4-butoxy-1-butanol (11.4%), gulopyranose (9.6%), lyxose (6.5%), 2,4-dimethyl-benzo[H]quinoline (5.1%), catechol (4.5%), and methoxyphenols (4.2%). The efficacy of the extract in controlling forest phytopathogens was tested in vitro against Fusarium circinatum (responsible for pitch canker of Pinus spp.), Cryphonectria parasitica (which causes chestnut blight), and Phytophthora cinnamomi (which causes ‘root and crown rot’ in a variety of hosts, including Castanea, conifers, Eucalyptus, Fagus, Juglans, Quercus, etc.), obtaining EC90 values of 322, 295, and 75 μg·mL−1, respectively, much lower than those attained for a commercial strobilurin fungicide (azoxystrobin). The extract was further tested ex situ against P. cinnamomi on artificially inoculated, excised stems of ‘Garnem’ almond rootstock, attaining complete protection at a dose of 782 μg·mL−1. The results suggest that holm oak bark extract may be a promising source of bioactive compounds against invasive forest pathogens, including the oomycete that is causing its decline, the so-called ‘seca’ in Spain.
000119974 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000119974 590__ $$a5.6$$b2022
000119974 592__ $$a1.154$$b2022
000119974 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b66 / 285 = 0.232$$c2022$$dQ1$$eT1
000119974 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1
000119974 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b52 / 178 = 0.292$$c2022$$dQ2$$eT1
000119974 593__ $$aPhysical and Theoretical Chemistry$$c2022$$dQ1
000119974 593__ $$aComputer Science Applications$$c2022$$dQ1
000119974 593__ $$aInorganic Chemistry$$c2022$$dQ1
000119974 593__ $$aSpectroscopy$$c2022$$dQ1
000119974 593__ $$aOrganic Chemistry$$c2022$$dQ1
000119974 593__ $$aMolecular Biology$$c2022$$dQ2
000119974 593__ $$aCatalysis$$c2022$$dQ2
000119974 594__ $$a7.8$$b2022
000119974 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000119974 700__ $$0(orcid)0000-0001-5136-8639$$aBalduque-Gil, Joaquín$$uUniversidad de Zaragoza
000119974 700__ $$aBarriuso-Vargas, Juan J.
000119974 700__ $$0(orcid)0000-0003-3009-0935$$aCasanova-Gascón, José$$uUniversidad de Zaragoza
000119974 700__ $$aGonzález-García, Vicente
000119974 700__ $$0(orcid)0000-0001-9067-2940$$aCuchí-Oterino, José Antonio$$uUniversidad de Zaragoza
000119974 700__ $$aLorenzo-Vidal, Belén
000119974 700__ $$aMartín-Gil, Jesús
000119974 700__ $$0(orcid)0000-0003-2713-2786$$aMartín-Ramos, Pablo$$uUniversidad de Zaragoza
000119974 7102_ $$15011$$2500$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cArea Ingeniería Agroforestal
000119974 7102_ $$15011$$2X$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cProy. investigación JFA
000119974 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000119974 773__ $$g23, 19 (2022), 11882 [16 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000119974 8564_ $$s1929103$$uhttps://zaguan.unizar.es/record/119974/files/texto_completo.pdf$$yVersión publicada
000119974 8564_ $$s2684807$$uhttps://zaguan.unizar.es/record/119974/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000119974 909CO $$ooai:zaguan.unizar.es:119974$$particulos$$pdriver
000119974 951__ $$a2024-03-18-16:40:07
000119974 980__ $$aARTICLE