000121401 001__ 121401
000121401 005__ 20240705134139.0
000121401 0247_ $$2doi$$a10.1038/s41598-022-04971-z
000121401 0248_ $$2sideral$$a131928
000121401 037__ $$aART-2022-131928
000121401 041__ $$aeng
000121401 100__ $$0(orcid)0000-0002-4665-9674$$aHerrera-Marcos, Luis V.$$uUniversidad de Zaragoza
000121401 245__ $$aHepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model
000121401 260__ $$c2022
000121401 5060_ $$aAccess copy available to the general public$$fUnrestricted
000121401 5203_ $$aNon-alcoholic fatty liver disease (NAFLD) is currently a growing epidemic disease that can lead to cirrhosis and hepatic cancer when it evolves into non-alcoholic steatohepatitis (NASH), a gap not well understood. To characterize this disease, pigs, considered to be one of the most similar to human experimental animal models, were used. To date, all swine-based settings have been carried out using rare predisposed breeds or long-term experiments. Herein, we fully describe a new experimental swine model for initial and reversible NASH using cross-bred animals fed on a high saturated fat, fructose, cholesterol, cholate, choline and methionine-deficient diet. To gain insight into the hepatic transcriptome that undergoes steatosis and steatohepatitis, we used RNA sequencing. This process significantly up-regulated 976 and down-regulated 209 genes mainly involved in cellular processes. Gene expression changes of 22 selected transcripts were verified by RT-qPCR. Lipid droplet area was positively associated with CD68, GPNMB, LGALS3, SLC51B and SPP1, and negatively with SQLE expressions. When these genes were tested in a second experiment of NASH reversion, LGALS3, SLC51B and SPP1 significantly decreased their expression. However, only LGALS3 was associated with lipid droplet areas. Our results suggest a role for LGALS3 in the transition of NAFLD to NASH.
000121401 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B16-20R$$9info:eu-repo/grantAgreement/ES/ISCIII-CIBERObn/CB06-03-1012$$9info:eu-repo/grantAgreement/ES/MICINN-ISCIIII-FEDER/PID2019-104915RB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R
000121401 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000121401 590__ $$a4.6$$b2022
000121401 592__ $$a0.973$$b2022
000121401 591__ $$aMULTIDISCIPLINARY SCIENCES$$b22 / 73 = 0.301$$c2022$$dQ2$$eT1
000121401 593__ $$aMultidisciplinary$$c2022$$dQ1
000121401 594__ $$a7.5$$b2022
000121401 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000121401 700__ $$0(orcid)0000-0002-8100-5596$$aMartínez-Beamonte, Roberto$$uUniversidad de Zaragoza
000121401 700__ $$aMacías-Herranz, Manuel
000121401 700__ $$0(orcid)0000-0003-1197-4276$$aArnal, Carmen$$uUniversidad de Zaragoza
000121401 700__ $$0(orcid)0000-0002-8442-8041$$aBarranquero, Cristina
000121401 700__ $$0(orcid)0000-0002-7213-7095$$aPuente-Lanzarote, Juan J.$$uUniversidad de Zaragoza
000121401 700__ $$aGascón, Sonia$$uUniversidad de Zaragoza
000121401 700__ $$aHerrero-Continente, Tania
000121401 700__ $$0(orcid)0009-0008-6478-7857$$aGonzalo-Romeo, Gonzalo$$uUniversidad de Zaragoza
000121401 700__ $$aAlastrué-Vera, Víctor
000121401 700__ $$aGutiérrez-Blázquez, Dolores
000121401 700__ $$0(orcid)0000-0001-9578-6525$$aLou-Bonafonte, José M.$$uUniversidad de Zaragoza
000121401 700__ $$0(orcid)0000-0002-5841-0462$$aSurra, Joaquín C.$$uUniversidad de Zaragoza
000121401 700__ $$0(orcid)0000-0002-3595-7668$$aRodríguez-Yoldi, María J.$$uUniversidad de Zaragoza
000121401 700__ $$0(orcid)0000-0002-8275-7191$$aGarcía-Gil, Agustín
000121401 700__ $$0(orcid)0000-0002-4437-2581$$aGüemes, Antonio$$uUniversidad de Zaragoza
000121401 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, Jesús$$uUniversidad de Zaragoza
000121401 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000121401 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000121401 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000121401 7102_ $$11013$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Cirugía
000121401 7102_ $$11009$$2X$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Técnica. Lab. y Talleres
000121401 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000121401 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000121401 773__ $$g12 (2022), 1024 [17 pp.]$$pSci. rep. (Nat. Publ. Group)$$tScientific reports (Nature Publishing Group)$$x2045-2322
000121401 8564_ $$s9999940$$uhttps://zaguan.unizar.es/record/121401/files/texto_completo.pdf$$yVersión publicada
000121401 8564_ $$s2560459$$uhttps://zaguan.unizar.es/record/121401/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000121401 909CO $$ooai:zaguan.unizar.es:121401$$particulos$$pdriver
000121401 951__ $$a2024-07-05-12:46:14
000121401 980__ $$aARTICLE