Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model

Herrera-Marcos, Luis V. (Universidad de Zaragoza) ; Martínez-Beamonte, Roberto (Universidad de Zaragoza) ; Macías-Herranz, Manuel ; Arnal, Carmen (Universidad de Zaragoza) ; Barranquero, Cristina ; Puente-Lanzarote, Juan J. (Universidad de Zaragoza) ; Gascón, Sonia (Universidad de Zaragoza) ; Herrero-Continente, Tania ; Gonzalo-Romeo, Gonzalo (Universidad de Zaragoza) ; Alastrué-Vera, Víctor ; Gutiérrez-Blázquez, Dolores ; Lou-Bonafonte, José M. (Universidad de Zaragoza) ; Surra, Joaquín C. (Universidad de Zaragoza) ; Rodríguez-Yoldi, María J. (Universidad de Zaragoza) ; García-Gil, Agustín ; Güemes, Antonio (Universidad de Zaragoza) ; Osada, Jesús (Universidad de Zaragoza)

Resumen: Non-alcoholic fatty liver disease (NAFLD) is currently a growing epidemic disease that can lead to cirrhosis and hepatic cancer when it evolves into non-alcoholic steatohepatitis (NASH), a gap not well understood. To characterize this disease, pigs, considered to be one of the most similar to human experimental animal models, were used. To date, all swine-based settings have been carried out using rare predisposed breeds or long-term experiments. Herein, we fully describe a new experimental swine model for initial and reversible NASH using cross-bred animals fed on a high saturated fat, fructose, cholesterol, cholate, choline and methionine-deficient diet. To gain insight into the hepatic transcriptome that undergoes steatosis and steatohepatitis, we used RNA sequencing. This process significantly up-regulated 976 and down-regulated 209 genes mainly involved in cellular processes. Gene expression changes of 22 selected transcripts were verified by RT-qPCR. Lipid droplet area was positively associated with CD68, GPNMB, LGALS3, SLC51B and SPP1, and negatively with SQLE expressions. When these genes were tested in a second experiment of NASH reversion, LGALS3, SLC51B and SPP1 significantly decreased their expression. However, only LGALS3 was associated with lipid droplet areas. Our results suggest a role for LGALS3 in the transition of NAFLD to NASH.

Idioma: Inglés

DOI: 10.1038/s41598-022-04971-z

Año: 2022

Publicado en: Scientific reports (Nature Publishing Group) 12 (2022), 1024 [17 pp.]
ISSN: 2045-2322

Factor impacto JCR: 4.6 (2022)

Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 22 / 73 = 0.301 (2022) - Q2 - T1

Factor impacto CITESCORE: 7.5 - General (Q1)

Factor impacto SCIMAGO: 0.973 - Multidisciplinary (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B16-20R
Financiación: info:eu-repo/grantAgreement/ES/ISCIII-CIBERObn/CB06-03-1012
Financiación: info:eu-repo/grantAgreement/ES/MICINN-ISCIIII-FEDER/PID2019-104915RB-I00
Financiación: info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Área Fisiología (Dpto. Farmac.Fisiol.y Med.L.F.)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)
Área (Departamento): Área Cirugía (Dpto. Cirugía)
Área (Departamento): Área Técnica. Lab. y Talleres (Dpto. Patología Animal)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Biología Celular (Dpto. Bioq.Biolog.Mol. Celular)