Dietary squalene supplementation decreases triglyceride species and modifies phospholipid lipidomic profile in the liver of a porcine model of non-alcoholic steatohepatitis

Herrera-Marcos, L. V. (Universidad de Zaragoza) ; Martínez-Beamonte, R. (Universidad de Zaragoza) ; Arnal, C. (Universidad de Zaragoza) ; Barranquero, C. ; Puente-Lanzarote, J. J. (Universidad de Zaragoza) ; Herrero-Continente, T. ; Lou-Bonafonte, J. M. (Universidad de Zaragoza) ; Gonzalo-Romeo, G. (Universidad de Zaragoza) ; Mocciaro, G. ; Jenkins, B. ; Surra, J. C. (Universidad de Zaragoza) ; Rodríguez-Yoldi, M. J. (Universidad de Zaragoza) ; Burillo, J. C. ; Lasheras, R. ; García-Gil, A. ; Güemes, A. (Universidad de Zaragoza) ; Koulman, A. ; Osada, J. (Universidad de Zaragoza)

Resumen: Squalene is a key minor component of virgin olive oil, the main source of fat in the Mediterranean diet, and had shown to improve the liver metabolism in rabbits and mice. The present research was carried out to find out whether this effect was conserved in a porcine model of hepatic steatohepatitis and to search for the lipidomic changes involved. The current study revealed that a 0.5% squalene supplementation to a steatotic diet for a month led to hepatic accumulation of squalene and decreased triglyceride content as well as area of hepatic lipid droplets without influencing cholesterol content or fiber areas. However, ballooning score was increased and associated with the hepatic squalene content. Of forty hepatic transcripts related to lipid metabolism and hepatic steatosis, only citrate synthase and a non-coding RNA showed decreased expressions. The hepatic lipidome, assessed by liquid chromatography-mass spectrometry in a platform able to analyze 467 lipids, revealed that squalene supplementation increased ceramide, Cer(36:2), and phosphatidylcholine (PC[32:0], PC[33:0] and PC[34:0]) species and decreased cardiolipin, CL(69:5), and triglyceride (TG[54:2], TG[55:0] and TG[55:2]) species. Plasma levels of interleukin 12p40 increased in pigs receiving the squalene diet. The latter also modified plasma lipidome by increasing TG(58:12) and decreasing non-esterified fatty acid (FA 14:0, FA 16:1 and FA 18:0) species without changes in total NEFA levels. Together this shows that squalene-induced changes in hepatic and plasma lipidomic profiles, non-coding RNA and anti-inflammatory interleukin are suggestive of an alleviation of the disease despite the increase in the ballooning score.

Idioma: Inglés

DOI: 10.1016/j.jnutbio.2022.109207

Año: 2023

Publicado en: Journal of Nutritional Biochemistry 112 (2023), 109207 [12 pp.]
ISSN: 0955-2863

Factor impacto JCR: 4.8 (2023)

Categ. JCR: NUTRITION & DIETETICS rank: 18 / 114 = 0.158 (2023) - Q1 - T1
Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 67 / 313 = 0.214 (2023) - Q1 - T1

Factor impacto CITESCORE: 9.5 - Molecular Biology (Q1) - Endocrinology, Diabetes and Metabolism (Q1) - Nutrition and Dietetics (Q1) - Clinical Biochemistry (Q1) - Biochemistry (Q1)

Factor impacto SCIMAGO: 1.136 - Biochemistry (Q1) - Clinical Biochemistry (Q1) - Nutrition and Dietetics (Q1) - Endocrinology, Diabetes and Metabolism (Q1) - Molecular Biology (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B16-20R
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/CB06-03-1012
Financiación: info:eu-repo/grantAgreement/ES/MICINN AEI/PID2019-104915RB-I00
Financiación: info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Área Fisiología (Dpto. Farmac.Fisiol.y Med.L.F.)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)
Área (Departamento): Área Cirugía (Dpto. Cirugía)
Área (Departamento): Serv. Div. Experimenta. Animal (Serv.Gral. Apoyo Investigación)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Biología Celular (Dpto. Bioq.Biolog.Mol. Celular)