000124034 001__ 124034 000124034 005__ 20241125101130.0 000124034 0247_ $$2doi$$a10.1016/j.jnutbio.2022.109207 000124034 0248_ $$2sideral$$a132636 000124034 037__ $$aART-2023-132636 000124034 041__ $$aeng 000124034 100__ $$0(orcid)0000-0002-4665-9674$$aHerrera-Marcos, L. V.$$uUniversidad de Zaragoza 000124034 245__ $$aDietary squalene supplementation decreases triglyceride species and modifies phospholipid lipidomic profile in the liver of a porcine model of non-alcoholic steatohepatitis 000124034 260__ $$c2023 000124034 5060_ $$aAccess copy available to the general public$$fUnrestricted 000124034 5203_ $$aSqualene is a key minor component of virgin olive oil, the main source of fat in the Mediterranean diet, and had shown to improve the liver metabolism in rabbits and mice. The present research was carried out to find out whether this effect was conserved in a porcine model of hepatic steatohepatitis and to search for the lipidomic changes involved. The current study revealed that a 0.5% squalene supplementation to a steatotic diet for a month led to hepatic accumulation of squalene and decreased triglyceride content as well as area of hepatic lipid droplets without influencing cholesterol content or fiber areas. However, ballooning score was increased and associated with the hepatic squalene content. Of forty hepatic transcripts related to lipid metabolism and hepatic steatosis, only citrate synthase and a non-coding RNA showed decreased expressions. The hepatic lipidome, assessed by liquid chromatography-mass spectrometry in a platform able to analyze 467 lipids, revealed that squalene supplementation increased ceramide, Cer(36:2), and phosphatidylcholine (PC[32:0], PC[33:0] and PC[34:0]) species and decreased cardiolipin, CL(69:5), and triglyceride (TG[54:2], TG[55:0] and TG[55:2]) species. Plasma levels of interleukin 12p40 increased in pigs receiving the squalene diet. The latter also modified plasma lipidome by increasing TG(58:12) and decreasing non-esterified fatty acid (FA 14:0, FA 16:1 and FA 18:0) species without changes in total NEFA levels. Together this shows that squalene-induced changes in hepatic and plasma lipidomic profiles, non-coding RNA and anti-inflammatory interleukin are suggestive of an alleviation of the disease despite the increase in the ballooning score. 000124034 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B16-20R$$9info:eu-repo/grantAgreement/ES/ISCIII/CB06-03-1012$$9info:eu-repo/grantAgreement/ES/MICINN AEI/PID2019-104915RB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R 000124034 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000124034 590__ $$a4.8$$b2023 000124034 592__ $$a1.136$$b2023 000124034 591__ $$aNUTRITION & DIETETICS$$b18 / 114 = 0.158$$c2023$$dQ1$$eT1 000124034 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b67 / 313 = 0.214$$c2023$$dQ1$$eT1 000124034 593__ $$aBiochemistry$$c2023$$dQ1 000124034 593__ $$aEndocrinology, Diabetes and Metabolism$$c2023$$dQ1 000124034 593__ $$aNutrition and Dietetics$$c2023$$dQ1 000124034 593__ $$aClinical Biochemistry$$c2023$$dQ1 000124034 593__ $$aMolecular Biology$$c2023$$dQ2 000124034 594__ $$a9.5$$b2023 000124034 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000124034 700__ $$0(orcid)0000-0002-8100-5596$$aMartínez-Beamonte, R.$$uUniversidad de Zaragoza 000124034 700__ $$0(orcid)0000-0003-1197-4276$$aArnal, C.$$uUniversidad de Zaragoza 000124034 700__ $$0(orcid)0000-0002-8442-8041$$aBarranquero, C. 000124034 700__ $$0(orcid)0000-0002-7213-7095$$aPuente-Lanzarote, J. J.$$uUniversidad de Zaragoza 000124034 700__ $$aHerrero-Continente, T. 000124034 700__ $$0(orcid)0000-0001-9578-6525$$aLou-Bonafonte, J. M.$$uUniversidad de Zaragoza 000124034 700__ $$0(orcid)0009-0008-6478-7857$$aGonzalo-Romeo, G.$$uUniversidad de Zaragoza 000124034 700__ $$aMocciaro, G. 000124034 700__ $$aJenkins, B. 000124034 700__ $$0(orcid)0000-0002-5841-0462$$aSurra, J. C.$$uUniversidad de Zaragoza 000124034 700__ $$0(orcid)0000-0002-3595-7668$$aRodríguez-Yoldi, M. J.$$uUniversidad de Zaragoza 000124034 700__ $$aBurillo, J. C. 000124034 700__ $$aLasheras, R. 000124034 700__ $$0(orcid)0000-0002-8275-7191$$aGarcía-Gil, A. 000124034 700__ $$0(orcid)0000-0002-4437-2581$$aGüemes, A.$$uUniversidad de Zaragoza 000124034 700__ $$aKoulman, A. 000124034 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, J.$$uUniversidad de Zaragoza 000124034 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000124034 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología 000124034 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal 000124034 7102_ $$11013$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Cirugía 000124034 7102_ $$10$$2X$$aUniversidad de Zaragoza$$bServ.Gral. Apoyo Investigación$$cServ. Div. Experimenta. Animal 000124034 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000124034 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular 000124034 773__ $$g112 (2023), 109207 [12 pp.]$$pJ. nutr. biochem.$$tJournal of Nutritional Biochemistry$$x0955-2863 000124034 8564_ $$s3417324$$uhttps://zaguan.unizar.es/record/124034/files/texto_completo.pdf$$yVersión publicada 000124034 8564_ $$s2497821$$uhttps://zaguan.unizar.es/record/124034/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000124034 909CO $$ooai:zaguan.unizar.es:124034$$particulos$$pdriver 000124034 951__ $$a2024-11-22-11:58:41 000124034 980__ $$aARTICLE