000124065 001__ 124065
000124065 005__ 20241125101131.0
000124065 0247_ $$2doi$$a10.1016/j.jcis.2022.11.138
000124065 0248_ $$2sideral$$a132462
000124065 037__ $$aART-2023-132462
000124065 041__ $$aeng
000124065 100__ $$aPaesa, Monica$$uUniversidad de Zaragoza
000124065 245__ $$aElucidating the mechanisms of action of antibiotic-like ionic gold and biogenic gold nanoparticles against bacteria
000124065 260__ $$c2023
000124065 5060_ $$aAccess copy available to the general public$$fUnrestricted
000124065 5203_ $$aThe antimicrobial action of gold depends on different factors including its oxidation state in the intra- and extracellular medium, the redox potential, its ability to produce reactive oxygen species (ROS), the medium components, the properties of the targeted bacteria wall, its penetration in the bacterial cytosol, the cell membrane potential, and its interaction with intracellular components. We demonstrate that different gold species are able to induce bacterial wall damage as a result of their electrostatic interaction with the cell membrane, the promotion of ROS generation, and the consequent DNA damage. In-depth genomic and proteomic studies on Escherichia coli confirmed the superior toxicity of Au (III) vs Au (I) based on the different molecular mechanisms analyzed including oxidative stress, bacterial energetic metabolism, biosynthetic processes, and cell transport. At equivalent bactericidal doses of Au (III) and Au (I) eukaryotic cells were not as affected as bacteria did, maintaining unaffected cell viability, morphology, and focal adhesions; however, increased ROS generation and disruption in the mitochondrial membrane potential were also observed. Herein, we shed light on the antimicrobial mechanisms of ionic and biogenic gold nanoparticles against bacteria. Under selected conditions antibiotic-like ionic gold can exert a strong antimicrobial activity while being harmless to human cells.
000124065 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-127847OB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-113987RB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/CTQ2017-84473-R$$9info:eu-repo/grantAgreement/ES/ISCIII/MS19-00092
000124065 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000124065 590__ $$a9.4$$b2023
000124065 592__ $$a1.76$$b2023
000124065 591__ $$aCHEMISTRY, PHYSICAL$$b32 / 178 = 0.18$$c2023$$dQ1$$eT1
000124065 593__ $$aBiomaterials$$c2023$$dQ1
000124065 593__ $$aSurfaces, Coatings and Films$$c2023$$dQ1
000124065 593__ $$aColloid and Surface Chemistry$$c2023$$dQ1
000124065 593__ $$aElectronic, Optical and Magnetic Materials$$c2023$$dQ1
000124065 594__ $$a16.1$$b2023
000124065 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000124065 700__ $$aRemirez de Ganuza, Cristina$$uUniversidad de Zaragoza
000124065 700__ $$0(orcid)0000-0002-9324-0446$$aAlejo, Teresa
000124065 700__ $$0(orcid)0000-0002-1505-498X$$aYus, Cristina$$uUniversidad de Zaragoza
000124065 700__ $$0(orcid)0000-0002-2966-9088$$aIrusta, Silvia$$uUniversidad de Zaragoza
000124065 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza
000124065 700__ $$0(orcid)0000-0002-6873-5244$$aSebastian, Víctor$$uUniversidad de Zaragoza
000124065 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, Gracia
000124065 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000124065 7102_ $$15005$$2790$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Tecnologi. Medio Ambiente
000124065 773__ $$g633 (2023), 786-799$$pJ. colloid interface sci.$$tJournal of Colloid and Interface Science$$x0021-9797
000124065 8564_ $$s3303498$$uhttps://zaguan.unizar.es/record/124065/files/texto_completo.pdf$$yVersión publicada
000124065 8564_ $$s2264329$$uhttps://zaguan.unizar.es/record/124065/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000124065 909CO $$ooai:zaguan.unizar.es:124065$$particulos$$pdriver
000124065 951__ $$a2024-11-22-11:58:58
000124065 980__ $$aARTICLE