Portable colorimetric enzymatic disposable biosensor for histamine and simultaneous histamine/tyramine determination using a smartphone

Sanz-Vicente, Isabel (Universidad de Zaragoza) ; Rivero, Irina ; Marcuello, Lucía ; Montano, María Pilar ; de Marcos, Susana (Universidad de Zaragoza) ; Galbán, Javier (Universidad de Zaragoza)

Resumen: Tyramine oxidase (TAO), peroxidase (HRP), and Amplex Red (AR) have been immobilized on cellulose to obtain disposable biosensors for the determination of histamine. During the enzymatic reaction, AR is oxidized and a pink spot is obtained. Using a smartphone and measuring the G (green) color coordinate, histamine can be determined in the presence of other biogenic amines (putrescine and cadaverine) in concentrations ranging from 2·10−5 M to 5·10−4 M with a 7.5·10−6 M limit of detection (LoD). Despite tyramine interference, experimental conditions are provided which allow rapid and simple histamine and simultaneous histamine/tyramine (semi)quantitative determination in mixtures. Finally, tyramine and histamine were determined in a tuna extract with good results (compared to the reference HPLC–MS method). The methodology can also be applied in solution allowing histamine (and simultaneous histamine/tyramine) determination with a lower LoD (1.8·10−7 M) and a similar selectivity.

Idioma: Inglés

DOI: 10.1007/s00216-023-04583-0

Año: 2023

Publicado en: Analytical and Bioanalytical Chemistry 415 (2023), 1777–1786
ISSN: 1618-2642

Factor impacto SCIMAGO: 0.686 - Biochemistry (Q2) - Analytical Chemistry (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E25-20R
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PDC2021-121224-I00
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2019-105408GB-I00

Tipo y forma: Article (Published version)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)