In Cellulo Bioorthogonal Catalysis by Encapsulated AuPd Nanoalloys: Overcoming Intracellular Deactivation

Rubio-Ruiz, Belén ; Pérez-López, Ana M. ; Uson, Laura ; Ortega-Liebana, M. Carmen ; Valero, Teresa ; Arruebo, Manuel (Universidad de Zaragoza) ; Hueso, Jose L. (Universidad de Zaragoza) ; Sebastian, Victor (Universidad de Zaragoza) ; Santamaria, Jesus (Universidad de Zaragoza) ; Unciti-Broceta, Asier

Resumen: Bioorthogonal metallocatalysis has opened up a xenobiotic route to perform nonenzymatic catalytic transformations in living settings. Despite their promising features, most metals are deactivated inside cells by a myriad of reactive biomolecules, including biogenic thiols, thereby limiting the catalytic functioning of these abiotic reagents. Here we report the development of cytocompatible alloyed AuPd nanoparticles with the capacity to elicit bioorthogonal depropargylations with high efficiency in biological media. We also show that the intracellular catalytic performance of these nanoalloys is significantly enhanced by protecting them following two different encapsulation methods. Encapsulation in mesoporous silica nanorods resulted in augmented catalyst reactivity, whereas the use of a biodegradable PLGA matrix increased nanoalloy delivery across the cell membrane. The functional potential of encapsulated AuPd was demonstrated by releasing the potent chemotherapy drug paclitaxel inside cancer cells. Nanoalloy encapsulation provides a novel methodology to develop nanoreactors capable of mediating new-to-life reactions in cells

Idioma: Inglés

DOI: 10.1021/acs.nanolett.2c03593

Año: 2023

Publicado en: Nano Letters 23, 3 (2023), 804-811
ISSN: 1530-6984

Factor impacto SCIMAGO: 3.411 - Bioengineering (Q1) - Chemistry (miscellaneous) (Q1) - Nanoscience and Nanotechnology (Q1) - Materials Science (miscellaneous) (Q1) - Mechanical Engineering (Q1) - Condensed Matter Physics (Q1)

Financiación: info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE
Financiación: info:eu-repo/grantAgreement/ES/MCIU/RTI2018-099019-A-I00

Tipo y forma: Article (Published version)

Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)