Identificación morfológica y molecular de parásitos Laelaps jettmari (mesostigmata: laelapidae) de pequeños roedores

Monguilod Brun, Paola
Kaminskienė, Evelina (dir.)

Universidad de Zaragoza, CIEN, 2022

Graduado en Biotecnología

Resumen: Information about Laelapidae mites infesting small rodents is scarce. In this study, we investigated morphological and molecular methods for identifying Laelaps jettmari mites, as well as carrying out phylogenetic analysis of these mites using nuclear rRNA 28S region (domains 1-3). Mites were first identified morphologically under the microscope. Afterwards, DNA was extracted using 2.5 % NH4OH solution and the rRNA 28S region (domains 1-3) was amplified by conventional PCR using the primers 43F and 929R. Then the PCR products were analysed by horizontal electrophoresis on 1.5% agarose gel and the positive samples were sent for sequencing to the sequencing centre in Macrogen, Amsterdam (Netherlands). After getting the sequence of L. jettmari, it was uploaded to MEGA X software for analysing it using evolutionary principles. The results of the study suggest that there is no difference between L. jettmari sequences collected from various rodent hosts in different geographical locations. The L. jettmari sequence obtained in this study and the sequences of Laelaps agilis collected from GenBank presented the shortest interspecific genetic distances of 28S rRNA region (domains 1-3), showing the slight evolutionary distance between these species. This paper gives novel molecular information for the identification and phylogenetic analysis of L.jettmari mites based on 28S rRNA region.

Tipo de Trabajo Académico: Trabajo Fin de Grado