000125150 001__ 125150
000125150 005__ 20230322092653.0
000125150 037__ $$aTAZ-TFG-2022-3046
000125150 041__ $$aeng
000125150 1001_ $$aMonguilod Brun, Paola
000125150 24200 $$aMorphological and molecular identification of Laelaps jettmari (mesostigmata: laelapidae) mites from small rodents
000125150 24500 $$aIdentificación morfológica y molecular de parásitos Laelaps jettmari (mesostigmata: laelapidae) de pequeños roedores
000125150 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2022
000125150 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/
000125150 520__ $$aInformation about Laelapidae mites infesting small rodents is scarce. In this study, we investigated morphological and molecular methods for identifying Laelaps jettmari mites, as well as carrying out phylogenetic analysis of these mites using nuclear rRNA 28S region (domains 1-3). Mites were first identified morphologically under the microscope. Afterwards, DNA was extracted using 2.5 % NH4OH solution and the rRNA 28S region (domains 1-3) was amplified by conventional PCR using the primers 43F and 929R. Then the PCR products were analysed by horizontal electrophoresis on 1.5% agarose gel and the positive samples were sent for sequencing to the sequencing centre in Macrogen, Amsterdam (Netherlands). After getting the sequence of L. jettmari, it was uploaded to MEGA X software for analysing it using evolutionary principles. The results of the study suggest that there is no difference between L. jettmari sequences collected from various rodent hosts in different geographical locations. The L. jettmari sequence obtained in this study and the sequences of Laelaps agilis collected from GenBank presented the shortest interspecific genetic distances of 28S rRNA region (domains 1-3), showing the slight evolutionary distance between these species. This paper gives novel molecular information for the identification and phylogenetic analysis of L.jettmari mites based on 28S rRNA region.<br /><br />
000125150 521__ $$aGraduado en Biotecnología
000125150 540__ $$aDerechos regulados por licencia Creative Commons
000125150 700__ $$aKaminskienė, Evelina$$edir.
000125150 7102_ $$aUniversidad de Zaragoza$$b $$c
000125150 8560_ $$f778631@unizar.es
000125150 8564_ $$s3041711$$uhttps://zaguan.unizar.es/record/125150/files/TAZ-TFG-2022-3046.pdf$$yMemoria (eng)
000125150 909CO $$ooai:zaguan.unizar.es:125150$$pdriver$$ptrabajos-fin-grado
000125150 950__ $$a
000125150 951__ $$adeposita:2023-03-21
000125150 980__ $$aTAZ$$bTFG$$cCIEN
000125150 999__ $$a20220701123843.CREATION_DATE