Tectomer-mediated optical nanosensors for tyramine determination

Domínguez, Mario (Universidad de Zaragoza) ; Oliver, Sofía ; Garriga, Rosa (Universidad de Zaragoza) ; Muñoz, Edgar ; Cebolla, Vicente L. ; de Marcos, Susana (Universidad de Zaragoza) ; Galbán, Javier (Universidad de Zaragoza)

Resumen: The development of optical sensors for in situ testing has become of great interest in the rapid diagnostics industry. We report here the development of simple, low-cost optical nanosensors for the semi-quantitative detection or naked-eye detection of tyramine (a biogenic amine whose production is commonly associated with food spoilage) when coupled to Au(III)/tectomer films deposited on polylactic acid (PLA) supports. Tectomers are two-dimensional oligoglycine self-assemblies, whose terminal amino groups enable both the immobilization of Au(III) and its adhesion to PLA. Upon exposure to tyramine, a non-enzymatic redox reaction takes place in which Au(III) in the tectomer matrix is reduced by tyramine to gold nanoparticles, whose reddish-purple color depends on the tyramine concentration and can be identified by measuring the RGB coordinates (Red–Green–Blue coordinates) using a smartphone color recognition app. Moreover, a more accurate quantification of tyramine in the range from 0.048 to 10 μM could be performed by measuring the reflectance of the sensing layers and the absorbance of the characteristic 550 nm plasmon band of the gold nanoparticles. The relative standard deviation (RSD) of the method was 4.2% (n = 5) with a limit of detection (LOD) of 0.014 μM. A remarkable selectivity was achieved for tyramine detection in the presence of other biogenic amines, especially histamine. This methodology, based on the optical properties of Au(III)/tectomer hybrid coatings, is promising for its application in food quality control and smart food packaging.

Idioma: Inglés

DOI: 10.3390/s23052524

Año: 2023

Publicado en: Sensors 23, 5 (2023), 2524 [11 pp.]
ISSN: 1424-8220

Factor impacto JCR: 3.4 (2023)

Categ. JCR: CHEMISTRY, ANALYTICAL rank: 34 / 106 = 0.321 (2023) - Q2 - T1
Categ. JCR: INSTRUMENTS & INSTRUMENTATION rank: 24 / 76 = 0.316 (2023) - Q2 - T1
Categ. JCR: ENGINEERING, ELECTRICAL & ELECTRONIC rank: 122 / 353 = 0.346 (2023) - Q2 - T2

Factor impacto CITESCORE: 7.3 - Instrumentation (Q1) - Information Systems (Q1) - Electrical and Electronic Engineering (Q1) - Analytical Chemistry (Q1) - Atomic and Molecular Physics, and Optics (Q1) - Biochemistry (Q2)

Factor impacto SCIMAGO: 0.786 - Instrumentation (Q1) - Analytical Chemistry (Q1) - Atomic and Molecular Physics, and Optics (Q1) - Information Systems (Q2) - Medicine (miscellaneous) (Q2) - Biochemistry (Q2) - Electrical and Electronic Engineering (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E25-20R
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PDC2021-121224-I00
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2019-105408GB-I00

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)
Área (Departamento): Área Química Física (Dpto. Química Física)