Evaluation of Nanopore sequencing for Mycobacterium tuberculosis drug susceptibility testing and outbreak investigation: a genomic analysis

Hall, Michael B. ; Rabodoarivelo, Marie Sylvianne (Universidad de Zaragoza) ; Koch, Anastasia ; Dippenaar, Anzaan ; George, Sophie ; Grobbelaar, Melanie ; Warren, Robin ; Walker, Timothy M. ; Cox, Helen ; Gagneux, Sebastien ; Crook, Derrick ; Peto, Tim ; Rakotosamimanana, Niaina ; Grandjean Lapierre, Simon ; Iqbal, Zamin

Resumen: Mycobacterium tuberculosis whole-genome sequencing (WGS) has been widely used for genotypic drug susceptibility testing (DST) and outbreak investigation. For both applications, Illumina technology is used by most public health laboratories; however, Nanopore technology developed by Oxford Nanopore Technologies has not been thoroughly evaluated. The aim of this study was to determine whether Nanopore sequencing data can provide equivalent information to Illumina for transmission clustering and genotypic DST for M tuberculosis.

Idioma: Inglés

DOI: 10.1016/S2666-5247(22)00301-9

Año: 2023

Publicado en: The Lancet Microbe 4, 2 (2023), e84-e92
ISSN: 2666-5247

Factor impacto JCR: 20.9 (2023)

Categ. JCR: MICROBIOLOGY rank: 3 / 161 = 0.019 (2023) - Q1 - T1
Categ. JCR: INFECTIOUS DISEASES rank: 2 / 132 = 0.015 (2023) - Q1 - T1

Factor impacto CITESCORE: 27.2 - Virology (Q1) - Infectious Diseases (Q1) - Microbiology (Q1) - Microbiology (medical) (Q1)

Factor impacto SCIMAGO: 5.392 - Infectious Diseases (Q1) - Virology (Q1) - Microbiology (medical) (Q1) - Microbiology (Q1)

Tipo y forma: Article (Published version)

Área (Departamento): Área Microbiología (Dpto. Microb.Ped.Radio.Sal.Pú.)