000125769 001__ 125769 000125769 005__ 20240731103326.0 000125769 0247_ $$2doi$$a10.1016/j.sbi.2023.102574 000125769 0248_ $$2sideral$$a133187 000125769 037__ $$aART-2023-133187 000125769 041__ $$aeng 000125769 100__ $$aGusach, A. 000125769 245__ $$aNew insights into GPCR coupling and dimerisation from cryo-EM structures 000125769 260__ $$c2023 000125769 5060_ $$aAccess copy available to the general public$$fUnrestricted 000125769 5203_ $$aOver the past three years (2020–2022) more structures of GPCRs have been determined than in the previous twenty years (2000–2019), primarily of GPCR complexes that are large enough for structure determination by single-particle cryo-EM. This review will present some structural highlights that have advanced our molecular understanding of promiscuous G protein coupling, how a G protein receptor kinase and β-arrestins couple to GPCRs, and GPCR dimerisation. We will also discuss advances in the use of gene fusions, nanobodies, and Fab fragments to facilitate the structure determination of GPCRs in the inactive state that, on their own, are too small for structure determination by single-particle cryo-EM. 000125769 536__ $$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/PID2020-113359GA-I00 000125769 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000125769 590__ $$a6.1$$b2023 000125769 592__ $$a2.824$$b2023 000125769 591__ $$aCELL BIOLOGY$$b48 / 205 = 0.234$$c2023$$dQ1$$eT1 000125769 593__ $$aStructural Biology$$c2023$$dQ1 000125769 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b47 / 313 = 0.15$$c2023$$dQ1$$eT1 000125769 593__ $$aMolecular Biology$$c2023$$dQ1 000125769 594__ $$a12.2$$b2023 000125769 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000125769 700__ $$0(orcid)0000-0002-4254-3148$$aGarcía-Nafría, J.$$uUniversidad de Zaragoza 000125769 700__ $$aTate, C. G. 000125769 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000125769 773__ $$g80 (2023), 102574 [10 pp.]$$pCurr. opin. struck. biol.$$tCurrent Opinion in Structural Biology$$x0959-440X 000125769 8564_ $$s2605019$$uhttps://zaguan.unizar.es/record/125769/files/texto_completo.pdf$$yVersión publicada 000125769 8564_ $$s2882931$$uhttps://zaguan.unizar.es/record/125769/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000125769 909CO $$ooai:zaguan.unizar.es:125769$$particulos$$pdriver 000125769 951__ $$a2024-07-31-09:44:53 000125769 980__ $$aARTICLE