Improvement of the seminal characteristics in rams using agri-food by-products rich in phytomelatonin

Peña-Delgado, Victoria (Universidad de Zaragoza) ; Carvajal-Serna, Melissa (Universidad de Zaragoza) ; Fondevila, Manuel (Universidad de Zaragoza) ; Martín-Cabrejas, María A. ; Aguilera, Yolanda ; Álvarez-Rivera, Gerardo ; Abecia, José A. (Universidad de Zaragoza) ; Casao, Adriana (Universidad de Zaragoza) ; Pérez-Pe, Rosaura (Universidad de Zaragoza)

Resumen: The aim of this study was to evaluate the effect of a phytomelatonin-rich diet, including by-products from the food industry, on ram sperm quality and seminal plasma composition. Melatonin content in several by-products before and after in vitro ruminal and abomasal digestion was determined by HPLC-ESI-MS/MS. Finally, 20% of a mix of grape pulp with pomegranate and tomato pomaces was included in the rams’ diet, constituting the phytomelatonin-rich diet. Feeding the rams with this diet resulted in an increase in seminal plasma melatonin levels compared with the control group (commercial diet) in the third month of the study. In addition, percentages higher than those in the control group of morphologically normal viable spermatozoa with a low content of reactive oxygen species were observed from the second month onwards. However, the antioxidant effect does not seem to be exerted through the modulation of the antioxidant enzymes since the analysis of the activities of catalase, glutathione reductase and glutathione peroxidase in seminal plasma revealed no significant differences between the two experimental groups. In conclusion, this study reveals, for the first time, that a phytomelatonin-rich diet can improve seminal characteristics in rams.

Idioma: Inglés

DOI: 10.3390/ani13050905

Año: 2023

Publicado en: Animals 13, 5 (2023), 905 [15 pp.]
ISSN: 2076-2615

Factor impacto JCR: 2.7 (2023)

Categ. JCR: VETERINARY SCIENCES rank: 16 / 167 = 0.096 (2023) - Q1 - T1
Categ. JCR: AGRICULTURE, DAIRY & ANIMAL SCIENCE rank: 10 / 80 = 0.125 (2023) - Q1 - T1

Factor impacto CITESCORE: 4.9 - Animal Science and Zoology (Q1) - Veterinary (all) (Q1)

Factor impacto SCIMAGO: 0.698 - Veterinary (miscellaneous) (Q1) - Animal Science and Zoology (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/A07-20R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2017-83799-R

Tipo y forma: Article (Published version)

Área (Departamento): Área Zoología (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)