000125861 001__ 125861 000125861 005__ 20240705134226.0 000125861 0247_ $$2doi$$a10.1021/acsami.2c20566 000125861 0248_ $$2sideral$$a133188 000125861 037__ $$aART-2023-133188 000125861 041__ $$aeng 000125861 100__ $$0(orcid)0000-0001-8762-5457$$aSancho-Albero, María$$uUniversidad de Zaragoza 000125861 245__ $$aSuperfluorinated extracellular vesicles for in vivo imaging by 19f-mri 000125861 260__ $$c2023 000125861 5060_ $$aAccess copy available to the general public$$fUnrestricted 000125861 5203_ $$aExtracellular vesicles (EVs) play a crucial role in cell-to-cell communication and have great potential as efficient delivery vectors. However, a better understanding of EV in vivo behavior is hampered by the limitations of current imaging tools. In addition, chemical labels present the risk of altering the EV membrane features and, thus, in vivo behavior. 19F-MRI is a safe bioimaging technique providing selective images of exogenous probes. Here, we present the first example of fluorinated EVs containing PERFECTA, a branched molecule with 36 magnetically equivalent 19F atoms. A PERFECTA emulsion is given to the cells, and PERFECTA-containing EVs are naturally produced. PERFECTA-EVs maintain the physicochemical features, morphology, and biological fingerprint as native EVs but exhibit an intense 19F-NMR signal and excellent 19F relaxation times. In vivo 19F-MRI and tumor-targeting capabilities of stem cell-derived PERFECTA-EVs are also proved. We propose PERFECTA-EVs as promising biohybrids for imaging biodistribution and delivery of EVs throughout the body. 000125861 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/CIBER-BBN$$9info:eu-repo/grantAgreement/ES/MEC/FPU15-03419$$9info:eu-repo/grantAgreement/ES/MINECO/Consolider-Ingenio2010 000125861 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000125861 592__ $$a2.058$$b2023 000125861 593__ $$aMaterials Science (miscellaneous)$$c2023$$dQ1 000125861 593__ $$aNanoscience and Nanotechnology$$c2023$$dQ1 000125861 593__ $$aMedicine (miscellaneous)$$c2023$$dQ1 000125861 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000125861 700__ $$aAyaz, Nazeeha 000125861 700__ $$0(orcid)0000-0002-6873-5244$$aSebastian, Victor$$uUniversidad de Zaragoza 000125861 700__ $$aChirizzi, Cristina 000125861 700__ $$aEncinas-Gimenez, Miguel$$uUniversidad de Zaragoza 000125861 700__ $$aNeri, Giulia 000125861 700__ $$aChaabane, Linda 000125861 700__ $$0(orcid)0000-0002-2053-9842$$aLuján, Lluís$$uUniversidad de Zaragoza 000125861 700__ $$aMartin-Duque, Pilar 000125861 700__ $$aMetrangolo, Pierangelo 000125861 700__ $$0(orcid)0000-0002-8701-9745$$aSantamaría, Jesús$$uUniversidad de Zaragoza 000125861 700__ $$aBaldelli Bombelli, Francesca 000125861 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000125861 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química 000125861 773__ $$g15 (2023), 8974-8985$$pACS appl. mater. interfaces$$tACS applied materials & interfaces$$x1944-8244 000125861 8564_ $$s9944759$$uhttps://zaguan.unizar.es/record/125861/files/texto_completo.pdf$$yVersión publicada 000125861 8564_ $$s2782234$$uhttps://zaguan.unizar.es/record/125861/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000125861 909CO $$ooai:zaguan.unizar.es:125861$$particulos$$pdriver 000125861 951__ $$a2024-07-05-12:55:45 000125861 980__ $$aARTICLE