Resumen: Professional self-concept in nurses is understood as the way nurses think and feel about themselves in their nursing role and is both a predictor of quality of care and a protective factor against burnout. The aim of this study was to translate, culturally adapt and validate the Spanish version of the Nurses Self-Concept Instrument in a sample of 483 Spanish registered nurses. In addition, we analyzed gender differences in its dimensions in the same sample. Internal reliability was evaluated using Cronbach’s Alpha, while construct validity was assessed using both exploratory and confirmatory factor analysis. The differences between groups were analyzed using the Mann-Whitney U test. Factor distribution was different from the original model. A gender gap was observed in the Nurse Thinking and Perception of Capabilities dimensions with higher values in the women group, while in the Leadership dimension, higher values were observed in the men group. While the Spanish version of the Nurses Self-Concept Instrument is a valid and reliable tool to measure this construct, the differences in its dimensions lead to a deeper understanding of the cultural differences in the construction of professional self-concept. Idioma: Inglés DOI: 10.3390/ijerph20021529 Año: 2023 Publicado en: International journal of environmental research and public health 20, 2 (2023), 1529 [11 pp.] ISSN: 1661-7827 Factor impacto CITESCORE: 7.3 - Health, Toxicology and Mutagenesis (Q1) - Public Health, Environmental and Occupational Health (Q1) - Pollution (Q2)