The E1a adenoviral gene upregulates the yamanaka factors to induce partial cellular reprogramming

Mendoza, Gracia ; González-Pastor, Rebeca ; Sánchez, Juan Miguel ; Arce-Cerezo, Altamira ; Quintanilla, Miguel ; Moreno-Bueno, Gema ; Pujol, Anna ; Belmar-López, Carolina ; de Martino, Alba ; Riu, Efrén ; Rodriguez, Tristan A. ; Martin-Duque, Pilar (Universidad de Zaragoza)

Resumen: The induction of pluripotency by enforced expression of different sets of genes in somatic cells has been achieved with reprogramming technologies first described by Yamanaka’s group. Methodologies for generating induced pluripotent stem cells are as varied as the combinations of genes used. It has previously been reported that the adenoviral E1a gene can induce the expression of two of the Yamanaka factors (c-Myc and Oct-4) and epigenetic changes. Here, we demonstrate that the E1a-12S over-expression is sufficient to induce pluripotent-like characteristics closely to epiblast stem cells in mouse embryonic fibroblasts through the activation of the pluripotency gene regulatory network. These findings provide not only empirical evidence that the expression of one single factor is sufficient for partial reprogramming but also a potential mechanistic explanation for how viral infection could lead to neoplasia if they are surrounded by the appropriate environment or the right medium, as happens with the tumorogenic niche.

Idioma: Inglés

DOI: 10.3390/cells12091338

Año: 2023

Publicado en: Cells 12, 9 (2023), 1338 [17 pp.]
ISSN: 2073-4409

Factor impacto JCR: 5.1 (2023)

Categ. JCR: CELL BIOLOGY rank: 63 / 205 = 0.307 (2023) - Q2 - T1

Factor impacto CITESCORE: 9.9 - Biochemistry, Genetics and Molecular Biology (all) (Q1)

Factor impacto SCIMAGO: 1.547 - Biochemistry, Genetics and Molecular Biology (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B054-12
Financiación: info:eu-repo/grantAgreement/ES/ISCIII DTS21-00130
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/PI19-01007

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Estomatología (Dpto. Cirugía)