Occurrence and limited zoonotic potential of Cryptosporidium spp., Giardia duodenalis, and Balantioides coli infections in free-ranging and farmed wild ungulates in Spain

Dashti, A. ; Köster, P. C. ; Bailo, B. ; Sánchez de las Matas, A. ; Habela, M. Á. ; Rivero-Juarez, A. ; Vicente, J. ; Serrano, E. ; Arnal, M. C. (Universidad de Zaragoza) ; Fernández de Luco, D. (Universidad de Zaragoza) ; Morrondo, P. ; Armenteros, J. A. ; Balseiro, A. ; Cardona, G. A. ; Martínez-Carrasco, C. ; Ortiz, J. A. ; Carpio, A. J. ; Calero-Bernal, R. ; González-Barrio, D. ; Carmena, D.

Resumen: Little information is currently available on the occurrence and molecular diversity of the enteric protozoan parasites Cryptosporidium spp., Giardia duodenalis, and Balantioides coli in wild ungulates and the role of these host species as potential sources of environmental contamination and consequent human infections. The presence of these three pathogens was investigated in eight wild ungulate species present in Spain (genera Ammotragus, Capra, Capreolus, Cervus, Dama, Ovis, Rupicapra, and Sus) by molecular methods. Faecal samples were retrospectively collected from free-ranging (n = 1058) and farmed (n = 324) wild ungulates from the five Spanish bioregions. Overall infection rates were 3.0% (42/1382; 95% CI: 2.1–3.9%) for Cryptosporidium spp., 5.4% (74/1382; 95% CI: 4.2–6.5%) for G. duodenalis, and 0.7% (9/1382; 95% CI: 0.3–1.2%) for B. coli. Cryptosporidium infection was detected in roe deer (7.5%), wild boar (7.0%) and red deer (1.5%), and G. duodenalis in southern chamois (12.9%), mouflon (10.0%), Iberian wild goat (9.0%), roe deer (7.5%), wild boar (5.6%), fallow deer (5.2%) and red deer (3.8%). Balantioides coli was only detected in wild boar (2.5%, 9/359). Sequence analyses revealed the presence of six distinct Cryptosporidium species: C. ryanae in red deer, roe deer, and wild boar; C. parvum in red deer and wild boar; C. ubiquitum in roe deer; C. scrofarum in wild boar; C. canis in roe deer; and C. suis in red deer. Zoonotic assemblages A and B were detected in wild boar and red deer, respectively. Ungulate-adapted assemblage E was identified in mouflon, red deer, and southern chamois. Attempts to genotype samples positive for B. coli failed. Sporadic infections by canine- or swine-adapted species may be indicative of potential cross-species transmission, although spurious infections cannot be ruled out. Molecular evidence gathered is consistent with parasite mild infections and limited environmental contamination with (oo)cysts. Free-ranging wild ungulate species would not presumably play a significant role as source of human infections by these pathogens. Wild ruminants do not seem to be susceptible hosts for B. coli.

Idioma: Inglés

DOI: 10.1016/j.rvsc.2023.04.020

Año: 2023

Publicado en: RESEARCH IN VETERINARY SCIENCE 159 (2023), 189-197
ISSN: 0034-5288

Factor impacto JCR: 2.2 (2023)

Categ. JCR: VETERINARY SCIENCES rank: 32 / 167 = 0.192 (2023) - Q1 - T1

Factor impacto SCIMAGO: 0.617 - Veterinary (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/CGL2016-80543-P
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/CD19-00011
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FI20-00002
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI19-00029
Financiación: info:eu-repo/grantAgreement/ES/MCIU/IJC-2020-042629-I
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CGL2012-40043-C02-01
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CGL2012-40043-C02-02
Financiación: info:eu-repo/grantAgreement/ES/MINECO/PID2020-115046GB-I00
Financiación: info:eu-repo/grantAgreement/ES/MINECO/RYC-2016-2020

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)