Granzyme-A deficiency attenuates experimental osteoarthritis in mice, but perforin deficiency does not
Calvo, Jorge (Universidad de Zaragoza) ; Santiago, Llipsy ; Arias, Maykel ; Pardo, Julián (Universidad de Zaragoza) ; Albareda, Jorge (Universidad de Zaragoza) ; Martínez-Lostao, Luis (Universidad de Zaragoza) ; García-Alvarez, Felícito (Universidad de Zaragoza)
Resumen: Objectives: This study aims to assess the development of osteoarthritis (OA) in granzyme A- (gzmA) and B- (gzmB) and perforin- (perf) knockout mice.
Materials and methods: A total of 75 male and female C57BL/6 (eight to nine-week-old) mice were allocated to: gzmA-deficient (gzmA-/-) (11 females, 8 males), gzmB-deficient (gzmB-/-) (9 females, 8 males), perf-deficient (perf-/-) (10 females, 9 males), and control group (10 females, 10 males). Osteoarthritis was induced in the right knee by instability of the meniscus medial ligament. Sham surgery was practiced in the left knee. Knee samples obtained eight weeks after surgery were stained (Safranin-O) and blindly scored in lateral and medial femur and tibia using the Osteoarthritis Research Society International scale (OARSI) (from Grade 0, cartilage intact to 6, deformation), (five stages from 0, no OA to 4, >50% surface involvement); OARSI score (grade x stage); and a semi-quantitative scale from Grade 0 (normal) to 6 (cartilage erosion >80%).
Results: Significantly higher values in all scales in the right knees compared to the left knees in male and female mice were observed (p<0.05). Males of all strains showed in the right knee higher values than females on all scales. Deficiency of perforin did not modify OA severity in any sex. The gzmA-/- females presented less degenerative changes than the other groups.
Conclusion: Our study results show that sex plays an important role in the development of experimental OA in mice. Deficiency of gzmA can protect from the development of OA in female mice.
Idioma: Inglés
DOI: 10.52312/jdrs.2023.892
Año: 2023
Publicado en: Joint diseases and related surgery 34, 2 (2023), 271-278
ISSN: 2687-4784
Factor impacto JCR: 1.9 (2023)
Categ. JCR: SURGERY rank: 119 / 292 = 0.408 (2023) - Q2 - T2
Categ. JCR: ORTHOPEDICS rank: 55 / 136 = 0.404 (2023) - Q2 - T2
Factor impacto CITESCORE: 2.5 - Rehabilitation (Q2) - Surgery (Q2) - Orthopedics and Sports Medicine (Q3)
Factor impacto SCIMAGO: 0.607 - Orthopedics and Sports Medicine (Q2) - Surgery (Q2) - Rehabilitation (Q2)
Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2020-113963RB-I00
Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/B29-17R
Tipo y forma: Article (Published version)
Área (Departamento): Área Traumatología y Ortopedia (Dpto. Cirugía)
Área (Departamento): Área Inmunología (Dpto. Microb.Ped.Radio.Sal.Pú.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes.
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Materials and methods: A total of 75 male and female C57BL/6 (eight to nine-week-old) mice were allocated to: gzmA-deficient (gzmA-/-) (11 females, 8 males), gzmB-deficient (gzmB-/-) (9 females, 8 males), perf-deficient (perf-/-) (10 females, 9 males), and control group (10 females, 10 males). Osteoarthritis was induced in the right knee by instability of the meniscus medial ligament. Sham surgery was practiced in the left knee. Knee samples obtained eight weeks after surgery were stained (Safranin-O) and blindly scored in lateral and medial femur and tibia using the Osteoarthritis Research Society International scale (OARSI) (from Grade 0, cartilage intact to 6, deformation), (five stages from 0, no OA to 4, >50% surface involvement); OARSI score (grade x stage); and a semi-quantitative scale from Grade 0 (normal) to 6 (cartilage erosion >80%).
Results: Significantly higher values in all scales in the right knees compared to the left knees in male and female mice were observed (p<0.05). Males of all strains showed in the right knee higher values than females on all scales. Deficiency of perforin did not modify OA severity in any sex. The gzmA-/- females presented less degenerative changes than the other groups.
Conclusion: Our study results show that sex plays an important role in the development of experimental OA in mice. Deficiency of gzmA can protect from the development of OA in female mice.
Idioma: Inglés
DOI: 10.52312/jdrs.2023.892
Año: 2023
Publicado en: Joint diseases and related surgery 34, 2 (2023), 271-278
ISSN: 2687-4784
Factor impacto JCR: 1.9 (2023)
Categ. JCR: SURGERY rank: 119 / 292 = 0.408 (2023) - Q2 - T2
Categ. JCR: ORTHOPEDICS rank: 55 / 136 = 0.404 (2023) - Q2 - T2
Factor impacto CITESCORE: 2.5 - Rehabilitation (Q2) - Surgery (Q2) - Orthopedics and Sports Medicine (Q3)
Factor impacto SCIMAGO: 0.607 - Orthopedics and Sports Medicine (Q2) - Surgery (Q2) - Rehabilitation (Q2)
Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2020-113963RB-I00
Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/B29-17R
Tipo y forma: Article (Published version)
Área (Departamento): Área Traumatología y Ortopedia (Dpto. Cirugía)
Área (Departamento): Área Inmunología (Dpto. Microb.Ped.Radio.Sal.Pú.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. Exportado de SIDERAL (2024-11-22-12:04:41)
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Este artículo se encuentra en las siguientes colecciones:
Articles > Artículos por área > Traumatología y Ortopedia
Articles > Artículos por área > Inmunología
Record created 2023-06-21, last modified 2024-11-25