Collagen-laponite nanoclay hydrogels for tumor spheroid growth

Alamán-Díez, P. (Universidad de Zaragoza) ; Borau, C. (Universidad de Zaragoza) ; Guerrero, P. E. (Universidad de Zaragoza) ; Amaveda, H. (Universidad de Zaragoza) ; Mora, M. (Universidad de Zaragoza) ; Fraile, J. M. ; García-Gareta, E. (Universidad de Zaragoza) ; García-Aznar, J. M. (Universidad de Zaragoza) ; Pérez, M. Á. (Universidad de Zaragoza)

Resumen: The extracellular matrix (ECM) plays an important regulatory role in the development and progression of tumoral tissue. Its functions and properties are crucial in determining tumor cell behavior such as invasion, migration, and malignancy development. Our study explores the role of collagen type I in cancer development and spread using engineered tumor models like multicellular spheroids grown in collagen-based hydrogels to simulate early tumor formation. We employ microfluidic techniques to test the hypothesis that (i) adding Laponite nanoclay to collagen hydrogels modifies mechanical and rheological properties and (ii) changing the stiffness of the collagen microenvironment affects tumor spheroid growth. Our findings support our theories and suggest the use of ECM components and engineered tumor models in cancer research, offering a biocompatible and biomimetic method to tailor the mechanical properties of conventional collagen hydrogels.

Idioma: Inglés

DOI: 10.1021/acs.biomac.3c00257

Año: 2023

Publicado en: BIOMACROMOLECULES 24, 6 (2023), 2879-2891
ISSN: 1525-7797

Factor impacto JCR: 5.5 (2023)

Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 58 / 313 = 0.185 (2023) - Q1 - T1
Categ. JCR: POLYMER SCIENCE rank: 10 / 94 = 0.106 (2023) - Q1 - T1
Categ. JCR: CHEMISTRY, ORGANIC rank: 3 / 58 = 0.052 (2023) - Q1 - T1

Factor impacto CITESCORE: 10.6 - Materials Chemistry (Q1) - Biomaterials (Q1) - Polymers and Plastics (Q1) - Bioengineering (Q1)

Factor impacto SCIMAGO: 1.232 - Bioengineering (Q1) - Polymers and Plastics (Q1) - Materials Chemistry (Q1) - Biomaterials (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/2018-22
Financiación: info:eu-repo/grantAgreement/EC/H2020/101018587/EU/Individual and Collective Migration of the Immune Cellular System/ICoMICS
Financiación: info:eu-repo/grantAgreement/EC/H2020/826494/EU/PRedictive In-silico Multiscale Analytics to support cancer personalized diaGnosis and prognosis, Empowered by imaging biomarkers/PRIMAGE
Financiación: info:eu-repo/grantAgreement/ES/MICINN/RYC2021-033490-I
Financiación: info:eu-repo/grantAgreement/ES/MINECO-AEI-FEDER/PID2021-122409OB-C21
Financiación: info:eu-repo/grantAgreement/ES/MINECO/PID2020-113819RB-I00
Financiación: info:eu-repo/grantAgreement/ES/MINECO/PID2020-125762NB-I00

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)
Área (Departamento): Área Cienc.Mater. Ingen.Metal. (Dpto. Ciencia Tecnol.Mater.Fl.)