000126591 001__ 126591 000126591 005__ 20241125101138.0 000126591 0247_ $$2doi$$a10.3390/ijms24087459 000126591 0248_ $$2sideral$$a133876 000126591 037__ $$aART-2023-133876 000126591 041__ $$aeng 000126591 100__ $$aPariente, Ana 000126591 245__ $$aIdentification of 7-Ketocholesterol-Modulated Pathways and Sterculic Acid Protective Effect in Retinal Pigmented Epithelium Cells by Using Genome-Wide Transcriptomic Analysis 000126591 260__ $$c2023 000126591 5060_ $$aAccess copy available to the general public$$fUnrestricted 000126591 5203_ $$aAge-related macular degeneration (AMD) is the leading cause of blindness in developed countries. AMD is characterized by the formation of lipidic deposits between the retinal pigment epithelium (RPE) and the choroid called drusen. 7-Ketocholesterol (7KCh), an oxidized-cholesterol derivative, is closely related to AMD as it is one of the main molecules accumulated in drusen. 7KCh induces inflammatory and cytotoxic responses in different cell types, and a better knowledge of the signaling pathways involved in its response would provide a new perspective on the molecular mechanisms that lead to the development of AMD. Furthermore, currently used therapies for AMD are not efficient enough. Sterculic acid (SA) attenuates the 7KCh response in RPE cells and is presented as an alternative to improve these therapies. By using genome-wide transcriptomic analysis in monkey RPE cells, we have provided new insight into 7KCh-induced signaling in RPE cells, as well as the protective capacity of SA. 7KCh modulates the expression of several genes associated with lipid metabolism, endoplasmic reticulum stress, inflammation and cell death and induces a complex response in RPE cells. The addition of SA successfully attenuates the deleterious effect of 7KCh and highlights its potential for the treatment of AMD. 000126591 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/CPII20-00029$$9info:eu-repo/grantAgreement/ES/ISCIII-FEDER/PI19/01805 000126591 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000126591 590__ $$a4.9$$b2023 000126591 592__ $$a1.179$$b2023 000126591 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b66 / 313 = 0.211$$c2023$$dQ1$$eT1 000126591 593__ $$aMedicine (miscellaneous)$$c2023$$dQ1 000126591 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b68 / 231 = 0.294$$c2023$$dQ2$$eT1 000126591 593__ $$aPhysical and Theoretical Chemistry$$c2023$$dQ1 000126591 593__ $$aComputer Science Applications$$c2023$$dQ1 000126591 593__ $$aInorganic Chemistry$$c2023$$dQ1 000126591 593__ $$aSpectroscopy$$c2023$$dQ1 000126591 593__ $$aOrganic Chemistry$$c2023$$dQ1 000126591 593__ $$aMolecular Biology$$c2023$$dQ2 000126591 593__ $$aCatalysis$$c2023$$dQ2 000126591 594__ $$a8.1$$b2023 000126591 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000126591 700__ $$aPérez-Sala, Álvaro 000126591 700__ $$aOchoa, Rodrigo 000126591 700__ $$aBobadilla, Miriam 000126591 700__ $$aVillanueva-Martínez, Ángela 000126591 700__ $$aPeláez, Rafael 000126591 700__ $$aLarráyoz, Ignacio M. 000126591 773__ $$g24, 8 (2023), 7459 [22 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596 000126591 8564_ $$s4327189$$uhttps://zaguan.unizar.es/record/126591/files/texto_completo.pdf$$yVersión publicada 000126591 8564_ $$s2781713$$uhttps://zaguan.unizar.es/record/126591/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000126591 909CO $$ooai:zaguan.unizar.es:126591$$particulos$$pdriver 000126591 951__ $$a2024-11-22-12:01:45 000126591 980__ $$aARTICLE