Radiographic and histopathological study of gastrointestinal dysmotility in lipopolysaccharide-induced sepsis in the rat

Castro, Marta (Universidad de Zaragoza) ; Valero, Marta Sofía (Universidad de Zaragoza) ; López-Tofiño, Yolanda ; López-Gómez, Laura ; Girón, Rocío ; Martín-Fontelles, María Isabel ; Uranga, José A. ; Abalo, Raquel

Resumen: AbstractSepsis is a highly incident condition in which a cascade of proinflammatory cytokines is involved. One of its most frequent consequences is ileus, which can increase mortality. Animal models such as that induced by systemic administration of lipopolysaccharide (LPS) are useful to deeply evaluate this condition. The effects of sepsis on the gastrointestinal (GI) tract have been explored but, to our knowledge, in vivo studies showing the motor and histopathological consequences of endotoxemia in an integrated way are lacking. Our aim was to study in rats the effects of sepsis on GI motility, using radiographic methods, and to assess histological damage in several organs.MethodsMale rats were intraperitoneally injected with saline or E. coli LPS at 0.1, 1, or 5 mg kg−1. Barium sulfate was intragastrically administered, and X‐rays were performed 0–24 h afterwards. Several organs were collected for organography, histopathology, and immunohistochemistry studies.Key ResultsAll LPS doses caused gastroparesia, whereas changes in intestinal motility were dose‐and time‐dependent, with an initial phase of hypermotility followed by paralytic ileus. Lung, liver, stomach, ileum, and colon (but not spleen or kidneys) were damaged, and density of neutrophils and activated M2 macrophages and expression of cyclooxygenase 2 were increased in the colon 24 h after LPS 5 mg kg−1.Conclusions and InferencesUsing radiographic, noninvasive methods for the first time, we show that systemic LPS causes dose‐, time‐, and organ‐dependent GI motor effects. Sepsis‐induced GI dysmotility is a complex condition whose management needs to take its time‐dependent changes into account.

Idioma: Inglés

DOI: 10.1111/nmo.14639

Año: 2023

Publicado en: NEUROGASTROENTEROLOGY AND MOTILITY 35, 10 (2023), e14639 [15 pp]
ISSN: 1350-1925

Factor impacto JCR: 3.5 (2023)

Categ. JCR: CLINICAL NEUROLOGY rank: 67 / 277 = 0.242 (2023) - Q1 - T1
Categ. JCR: NEUROSCIENCES rank: 109 / 310 = 0.352 (2023) - Q2 - T2
Categ. JCR: GASTROENTEROLOGY & HEPATOLOGY rank: 43 / 143 = 0.301 (2023) - Q2 - T1

Factor impacto CITESCORE: 7.8 - Gastroenterology (Q1) - Endocrine and Autonomic Systems (Q1) - Physiology (Q1)

Factor impacto SCIMAGO: 1.312 - Endocrine and Autonomic Systems (Q1) - Physiology (Q1) - Gastroenterology (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B04-17R
Financiación: info:eu-repo/grantAgreement/ES/MICINN/SAF2012-40075-C02-01
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2017-82987-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/SAF2017-83120-C2-1-R
Financiación: info:eu-repo/grantAgreement/ES/UZ/JIUZ-2015-BIO-02

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Fisiología (Dpto. Farmac.Fisiol.y Med.L.F.)