000127027 001__ 127027
000127027 005__ 20241125101156.0
000127027 0247_ $$2doi$$a10.1111/nmo.14639
000127027 0248_ $$2sideral$$a134329
000127027 037__ $$aART-2023-134329
000127027 041__ $$aeng
000127027 100__ $$0(orcid)0000-0001-8584-3979$$aCastro, Marta$$uUniversidad de Zaragoza
000127027 245__ $$aRadiographic and histopathological study of gastrointestinal dysmotility in lipopolysaccharide-induced sepsis in the rat
000127027 260__ $$c2023
000127027 5060_ $$aAccess copy available to the general public$$fUnrestricted
000127027 5203_ $$aAbstractSepsis is a highly incident condition in which a cascade of proinflammatory cytokines is involved. One of its most frequent consequences is ileus, which can increase mortality. Animal models such as that induced by systemic administration of lipopolysaccharide (LPS) are useful to deeply evaluate this condition. The effects of sepsis on the gastrointestinal (GI) tract have been explored but, to our knowledge, in vivo studies showing the motor and histopathological consequences of endotoxemia in an integrated way are lacking. Our aim was to study in rats the effects of sepsis on GI motility, using radiographic methods, and to assess histological damage in several organs.MethodsMale rats were intraperitoneally injected with saline or E. coli LPS at 0.1, 1, or 5 mg kg−1. Barium sulfate was intragastrically administered, and X‐rays were performed 0–24 h afterwards. Several organs were collected for organography, histopathology, and immunohistochemistry studies.Key ResultsAll LPS doses caused gastroparesia, whereas changes in intestinal motility were dose‐and time‐dependent, with an initial phase of hypermotility followed by paralytic ileus. Lung, liver, stomach, ileum, and colon (but not spleen or kidneys) were damaged, and density of neutrophils and activated M2 macrophages and expression of cyclooxygenase 2 were increased in the colon 24 h after LPS 5 mg kg−1.Conclusions and InferencesUsing radiographic, noninvasive methods for the first time, we show that systemic LPS causes dose‐, time‐, and organ‐dependent GI motor effects. Sepsis‐induced GI dysmotility is a complex condition whose management needs to take its time‐dependent changes into account.
000127027 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B04-17R$$9info:eu-repo/grantAgreement/ES/MICINN/SAF2012-40075-C02-01$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-82987-R$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2017-83120-C2-1-R$$9info:eu-repo/grantAgreement/ES/UZ/JIUZ-2015-BIO-02
000127027 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000127027 590__ $$a3.5$$b2023
000127027 592__ $$a1.312$$b2023
000127027 591__ $$aCLINICAL NEUROLOGY$$b70 / 280 = 0.25$$c2023$$dQ1$$eT1
000127027 593__ $$aEndocrine and Autonomic Systems$$c2023$$dQ1
000127027 591__ $$aNEUROSCIENCES$$b110 / 310 = 0.355$$c2023$$dQ2$$eT2
000127027 593__ $$aPhysiology$$c2023$$dQ1
000127027 591__ $$aGASTROENTEROLOGY & HEPATOLOGY$$b43 / 143 = 0.301$$c2023$$dQ2$$eT1
000127027 593__ $$aGastroenterology$$c2023$$dQ1
000127027 594__ $$a7.8$$b2023
000127027 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000127027 700__ $$0(orcid)0000-0002-2231-7565$$aValero, Marta Sofía$$uUniversidad de Zaragoza
000127027 700__ $$aLópez-Tofiño, Yolanda
000127027 700__ $$aLópez-Gómez, Laura
000127027 700__ $$aGirón, Rocío
000127027 700__ $$aMartín-Fontelles, María Isabel
000127027 700__ $$aUranga, José A.
000127027 700__ $$aAbalo, Raquel
000127027 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000127027 773__ $$g35, 10 (2023), e14639 [15 pp]$$pNeurogastroenterol. motil.$$tNEUROGASTROENTEROLOGY AND MOTILITY$$x1350-1925
000127027 8564_ $$s4378878$$uhttps://zaguan.unizar.es/record/127027/files/texto_completo.pdf$$yVersión publicada
000127027 8564_ $$s2366268$$uhttps://zaguan.unizar.es/record/127027/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000127027 909CO $$ooai:zaguan.unizar.es:127027$$particulos$$pdriver
000127027 951__ $$a2024-11-22-12:09:36
000127027 980__ $$aARTICLE