A novel introgression line library derived from a wild melon gives insights into the genetics of melon domestication, uncovering new genetic variability useful for breeding

Campos, Manuel ; Gonzalo, Maria José ; Díaz, Aurora (Universidad de Zaragoza) ; Picó, Belén ; Gómez-Guillamón, Maria Luisa ; Monforte, Antonio José ; Esteras, Cristina

Resumen: A collection of 30 melon introgression lines (ILs) was developed from the wild accession Ames 24297 (TRI) into ‘Piel de Sapo’ (PS) genetic background. Each IL carried an average of 1.4 introgressions from TRI, and the introgressions represented 91.4% of the TRI genome. Twenty-two ILs, representing 75% of the TRI genome, were evaluated in greenhouse (Algarrobo and Meliana) and field (Alcàsser) trials, mainly to study traits related to domestication syndrome such as fruit weight (FW) and flesh content (FFP), as well as other fruit quality traits as fruit shape (FS), flesh firmness (FF), soluble solid concentration (SSC), rind color and abscission layer. The IL collection showed an impressive variation in size-related traits, with FW ranging from 800 to 4100 g, reflecting the strong effect of the wild genome on these traits. Most of the ILs produced smaller fruits compared with PS; however, unexpectedly, the IL TRI05-2 produced bigger fruits, likely due to new epistatic interacions with the PS genetic background. In contrast, the genotypic effect for FS was smaller, and few QTLs with notable effects were detected. Interestingly, variability was also observed for FFP, FF and SSC, rind color and abscission layer formation. Genes in these introgressions are candidates for having been involved in melon domestication and diversification as well. These results confirm that the TRI IL collection is a very powerful tool for mapping traits of agronomic interest in melon, allowing the confirmation of previously reported QTLs and the identification of new ones to better understand the domestication process of this crop.

Idioma: Inglés

DOI: 10.3390/ijms241210099

Año: 2023

Publicado en: International Journal of Molecular Sciences 24, 12 (2023), 10099 [18 pp.]
ISSN: 1661-6596

Factor impacto JCR: 4.9 (2023)

Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 66 / 313 = 0.211 (2023) - Q1 - T1
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 68 / 231 = 0.294 (2023) - Q2 - T1

Factor impacto CITESCORE: 8.1 - Spectroscopy (Q1) - Computer Science Applications (Q1) - Physical and Theoretical Chemistry (Q1) - Inorganic Chemistry (Q1) - Organic Chemistry (Q1) - Molecular Biology (Q2) - Catalysis (Q2)

Factor impacto SCIMAGO: 1.179 - Medicine (miscellaneous) (Q1) - Physical and Theoretical Chemistry (Q1) - Computer Science Applications (Q1) - Inorganic Chemistry (Q1) - Spectroscopy (Q1) - Organic Chemistry (Q1) - Molecular Biology (Q2) - Catalysis (Q2)

Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2020-116055RB-C22
Financiación: info:eu-repo/grantAgreement/ES/MCIU-FEDER/RTI2018-097665-B-C22
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2015-64625-C2-2-R

Tipo y forma: Article (Published version)

Área (Departamento): Área Producción Vegetal (Dpto. CC.Agrar.y Medio Natural)