The Mechanical and Biological Performance of Photopolymerized Gelatin-Based Hydrogels as a Function of the Reaction Media

Pamplona, Regina ; González-Lana, Sandra ; Romero, Pilar ; Ochoa, Ignacio (Universidad de Zaragoza) ; Martín-Rapún, Rafael (Universidad de Zaragoza) ; Sánchez-Somolinos, Carlos

Resumen: From the first experiments with biomaterials to mimic tissue properties, the mechanical and biochemical characterization has evolved extensively. Several properties can be described, however, what should be essential is to conduct a proper and physiologically relevant characterization. Herein, the influence of the reaction media (RM) and swelling media (SM)–phosphate buffered saline (PBS) and Dulbecco's modified Eagle's medium (DMEM) with two different glucose concentrations–is described in gelatin methacrylamide (GelMA) hydrogel mechanics and in the biological behavior of two tumoral cell lines (Caco‐2 and HCT‐116). All scaffolds are UV‐photocrosslinked under identical conditions and evaluated for mass swelling ratio and stiffness. The results indicate that stiffness is highly susceptible to the RM, but not to the SM. Additionally, PBS‐prepared hydrogels exhibited a higher photopolymerization degree according to high resolution magic‐angle spinning (HR‐MAS) NMR. These findings correlate with the biological response of Caco‐2 and HCT‐116 cells seeded on the substrates, which demonstrated flatter morphologies on stiffer hydrogels. Overall, cell viability and proliferation are excellent for both cell lines, and Caco‐2 cells displayed a characteristic apical‐basal polarization based on F‐actin/Nuclei fluorescence images. These characterization experiments highlight the importance of conducting mechanical testing of biomaterials in the same medium as cell culture.

Idioma: Inglés

DOI: 10.1002/mabi.202300227

Año: 2023

Publicado en: Macromolecular Bioscience 23, 12 (2023), 2300227 [13 pp.]
ISSN: 1616-5187

Factor impacto JCR: 4.4 (2023)

Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 83 / 313 = 0.265 (2023) - Q2 - T1
Categ. JCR: POLYMER SCIENCE rank: 24 / 95 = 0.253 (2023) - Q2 - T1
Categ. JCR: MATERIALS SCIENCE, BIOMATERIALS rank: 23 / 53 = 0.434 (2023) - Q2 - T2

Factor impacto CITESCORE: 7.9 - Biotechnology (Q1) - Polymers and Plastics (Q1) - Materials Chemistry (Q1) - Biomaterials (Q2) - Bioengineering (Q2)

Factor impacto SCIMAGO: 0.907 - Biotechnology (Q1) - Materials Chemistry (Q1) - Polymers and Plastics (Q1) - Biomaterials (Q2) - Bioengineering (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E15-20R
Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/LMP221_21
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2020-118485RB-I00
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2021-126132NB-I00
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DI2017-09585

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Area Histología (Dpto. Anatom.Histolog.Humanas)
Área (Departamento): Área Química Orgánica (Dpto. Química Orgánica)