000127963 001__ 127963
000127963 005__ 20241125101158.0
000127963 0247_ $$2doi$$a10.1002/mabi.202300227
000127963 0248_ $$2sideral$$a135010
000127963 037__ $$aART-2023-135010
000127963 041__ $$aeng
000127963 100__ $$0(orcid)0000-0002-1647-8207$$aPamplona, Regina
000127963 245__ $$aThe Mechanical and Biological Performance of Photopolymerized Gelatin-Based Hydrogels as a Function of the Reaction Media
000127963 260__ $$c2023
000127963 5060_ $$aAccess copy available to the general public$$fUnrestricted
000127963 5203_ $$aFrom the first experiments with biomaterials to mimic tissue properties, the mechanical and biochemical characterization has evolved extensively. Several properties can be described, however, what should be essential is to conduct a proper and physiologically relevant characterization. Herein, the influence of the reaction media (RM) and swelling media (SM)–phosphate buffered saline (PBS) and Dulbecco's modified Eagle's medium (DMEM) with two different glucose concentrations–is described in gelatin methacrylamide (GelMA) hydrogel mechanics and in the biological behavior of two tumoral cell lines (Caco‐2 and HCT‐116). All scaffolds are UV‐photocrosslinked under identical conditions and evaluated for mass swelling ratio and stiffness. The results indicate that stiffness is highly susceptible to the RM, but not to the SM. Additionally, PBS‐prepared hydrogels exhibited a higher photopolymerization degree according to high resolution magic‐angle spinning (HR‐MAS) NMR. These findings correlate with the biological response of Caco‐2 and HCT‐116 cells seeded on the substrates, which demonstrated flatter morphologies on stiffer hydrogels. Overall, cell viability and proliferation are excellent for both cell lines, and Caco‐2 cells displayed a characteristic apical‐basal polarization based on F‐actin/Nuclei fluorescence images. These characterization experiments highlight the importance of conducting mechanical testing of biomaterials in the same medium as cell culture.
000127963 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E15-20R$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/LMP221_21$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-118485RB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-126132NB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/DI2017-09585
000127963 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000127963 590__ $$a4.4$$b2023
000127963 592__ $$a0.907$$b2023
000127963 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b83 / 313 = 0.265$$c2023$$dQ2$$eT1
000127963 593__ $$aBiotechnology$$c2023$$dQ1
000127963 591__ $$aPOLYMER SCIENCE$$b24 / 95 = 0.253$$c2023$$dQ2$$eT1
000127963 593__ $$aMaterials Chemistry$$c2023$$dQ1
000127963 591__ $$aMATERIALS SCIENCE, BIOMATERIALS$$b23 / 53 = 0.434$$c2023$$dQ2$$eT2
000127963 593__ $$aPolymers and Plastics$$c2023$$dQ1
000127963 593__ $$aBiomaterials$$c2023$$dQ2
000127963 593__ $$aBioengineering$$c2023$$dQ2
000127963 594__ $$a7.9$$b2023
000127963 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000127963 700__ $$aGonzález-Lana, Sandra
000127963 700__ $$0(orcid)0000-0003-1378-0571$$aRomero, Pilar
000127963 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa, Ignacio$$uUniversidad de Zaragoza
000127963 700__ $$0(orcid)0000-0003-0702-8260$$aMartín-Rapún, Rafael$$uUniversidad de Zaragoza
000127963 700__ $$0(orcid)0000-0003-3900-2866$$aSánchez-Somolinos, Carlos
000127963 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000127963 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000127963 773__ $$g23, 12 (2023), 2300227 [13 pp.]$$pMacromol. biosci.$$tMacromolecular Bioscience$$x1616-5187
000127963 8564_ $$s4434597$$uhttps://zaguan.unizar.es/record/127963/files/texto_completo.pdf$$yVersión publicada
000127963 8564_ $$s2423990$$uhttps://zaguan.unizar.es/record/127963/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000127963 909CO $$ooai:zaguan.unizar.es:127963$$particulos$$pdriver
000127963 951__ $$a2024-11-22-12:10:20
000127963 980__ $$aARTICLE