Increased circulating microRNAs miR-342-3p and miR-21-5p in natural sheep prion disease

Sanz Rubio, D ; López-Pérez, O (Universidad de Zaragoza) ; de Andrés Pablo, A ; Bolea, R (Universidad de Zaragoza) ; Osta, R (Universidad de Zaragoza) ; Badiola, JJ (Universidad de Zaragoza) ; Zaragoza, P (Universidad de Zaragoza) ; Martín-Burriel, I (Universidad de Zaragoza) ; Toivonen, JM. (Universidad de Zaragoza)

Resumen: Scrapie is a transmissible spongiform encephalopathy (TSE), or prion disease, of sheep and goats. As no simple diagnostic tests are yet available to detect TSEs in vivo, easily accessible biomarkers could facilitate the eradication of scrapie agents from the food chain. To this end, we analysed by quantitative reverse transcription PCR a selected set of candidate microRNAs (miRNAs) from circulating blood plasma of naturally infected, classical scrapie sheep that demonstrated clear scrapie symptoms and pathology. Significant scrapie-associated increase was repeatedly found for miR-342-3p and miR-21-5p. This is the first demonstration, to our knowledge, of circulating miRNA alterations in any animal suffering from TSE. Genome-wide expression studies are warranted to investigate the true depth of miRNA alterations in naturally occurring TSEs, especially in presymptomatic animals, as the presented study demonstrates the potential feasibility of miRNAs as circulating TSE biomarkers.

Idioma: Inglés

DOI: 10.1099/jgv.0.000685

Año: 2017

Publicado en: JOURNAL OF GENERAL VIROLOGY 98 (2017), 305-310
ISSN: 0022-1317

Factor impacto JCR: 2.514 (2017)

Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 66 / 159 = 0.415 (2017) - Q2 - T2
Categ. JCR: VIROLOGY rank: 18 / 35 = 0.514 (2017) - Q3 - T2

Factor impacto SCIMAGO: 1.325 - Virology (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/A17
Financiación: info:eu-repo/grantAgreement/ES/UZ/UZ2014-BIO-5

Tipo y forma: Article (Published version)

Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)