000129543 001__ 129543
000129543 005__ 20231221152907.0
000129543 0247_ $$2doi$$a10.1371/journal.pone.0051058
000129543 0248_ $$2sideral$$a80133
000129543 037__ $$aART-2012-80133
000129543 041__ $$aeng
000129543 100__ $$0(orcid)0000-0002-7734-8481$$aLópez-Alvarez, D.$$uUniversidad de Zaragoza
000129543 245__ $$aA DNA Barcoding Method to Discriminate between the Model Plant Brachypodium distachyon and Its Close Relatives B. stacei and B. hybridum (Poaceae)
000129543 260__ $$c2012
000129543 5060_ $$aAccess copy available to the general public$$fUnrestricted
000129543 5203_ $$aBackground
Brachypodium distachyon s. l. has been widely investigated across the world as a model plant for temperate cereals and biofuel grasses. However, this annual plant shows three cytotypes that have been recently recognized as three independent species, the diploids B. distachyon (2n = 10) and B. stacei (2n = 20) and their derived allotetraploid B. hybridum (2n = 30).
Methodology/Principal Findings
We propose a DNA barcoding approach that consists of a rapid, accurate and automatable species identification method using the standard DNA sequences of complementary plastid (trnLF) and nuclear (ITS, GI) loci. The highly homogenous but largely divergent B. distachyon and B. stacei diploids could be easily distinguished (100% identification success) using direct trnLF (2.4%), ITS (5.5%) or GI (3.8%) sequence divergence. By contrast, B. hybridum could only be unambiguously identified through the use of combined trnLF+ITS sequences (90% of identification success) or by cloned GI sequences (96.7%) that showed 5.4% (ITS) and 4% (GI) rate divergence between the two parental sequences found in the allopolyploid.
Conclusion/Significance
Our data provide an unbiased and effective barcode to differentiate these three closely-related species from one another. This procedure overcomes the taxonomic uncertainty generated from methods based on morphology or flow cytometry identifications that have resulted in some misclassifications of the model plant and its allies. Our study also demonstrates that the allotetraploid B. hybridum has resulted from bi-directional crosses of B. distachyon and B. stacei plants acting either as maternal or paternal parents.
000129543 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/Bioflora$$9info:eu-repo/grantAgreement/ES/MICINN/CGL2009-12955-C02-01
000129543 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000129543 590__ $$a3.73$$b2012
000129543 591__ $$aMULTIDISCIPLINARY SCIENCES$$b7 / 57 = 0.123$$c2012$$dQ1$$eT1
000129543 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000129543 700__ $$aLópez-Herranz, M. L.
000129543 700__ $$aBetekhtin, A.
000129543 700__ $$0(orcid)0000-0001-7793-5259$$aCatalán, P.$$uUniversidad de Zaragoza
000129543 7102_ $$15011$$2063$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Botánica
000129543 773__ $$g7, 12 (2012), e51058 [20 pp.]$$pPLoS One$$tPLoS ONE$$x1932-6203
000129543 8564_ $$s636602$$uhttps://zaguan.unizar.es/record/129543/files/texto_completo.pdf$$yVersión publicada
000129543 8564_ $$s3210174$$uhttps://zaguan.unizar.es/record/129543/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000129543 909CO $$ooai:zaguan.unizar.es:129543$$particulos$$pdriver
000129543 951__ $$a2023-12-21-13:08:18
000129543 980__ $$aARTICLE