A DNA Barcoding Method to Discriminate between the Model Plant Brachypodium distachyon and Its Close Relatives B. stacei and B. hybridum (Poaceae)
López-Alvarez, D. (Universidad de Zaragoza) ; López-Herranz, M. L. ; Betekhtin, A. ; Catalán, P. (Universidad de Zaragoza)
Resumen: Background
Brachypodium distachyon s. l. has been widely investigated across the world as a model plant for temperate cereals and biofuel grasses. However, this annual plant shows three cytotypes that have been recently recognized as three independent species, the diploids B. distachyon (2n = 10) and B. stacei (2n = 20) and their derived allotetraploid B. hybridum (2n = 30).
Methodology/Principal Findings
We propose a DNA barcoding approach that consists of a rapid, accurate and automatable species identification method using the standard DNA sequences of complementary plastid (trnLF) and nuclear (ITS, GI) loci. The highly homogenous but largely divergent B. distachyon and B. stacei diploids could be easily distinguished (100% identification success) using direct trnLF (2.4%), ITS (5.5%) or GI (3.8%) sequence divergence. By contrast, B. hybridum could only be unambiguously identified through the use of combined trnLF+ITS sequences (90% of identification success) or by cloned GI sequences (96.7%) that showed 5.4% (ITS) and 4% (GI) rate divergence between the two parental sequences found in the allopolyploid.
Conclusion/Significance
Our data provide an unbiased and effective barcode to differentiate these three closely-related species from one another. This procedure overcomes the taxonomic uncertainty generated from methods based on morphology or flow cytometry identifications that have resulted in some misclassifications of the model plant and its allies. Our study also demonstrates that the allotetraploid B. hybridum has resulted from bi-directional crosses of B. distachyon and B. stacei plants acting either as maternal or paternal parents.
Idioma: Inglés
DOI: 10.1371/journal.pone.0051058
Año: 2012
Publicado en: PLoS ONE 7, 12 (2012), e51058 [20 pp.]
ISSN: 1932-6203
Factor impacto JCR: 3.73 (2012)
Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 7 / 57 = 0.123 (2012) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/Bioflora
Financiación: info:eu-repo/grantAgreement/ES/MICINN/CGL2009-12955-C02-01
Tipo y forma: Article (Published version)
Área (Departamento): Área Botánica (Dpto. CC.Agrar.y Medio Natural)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Exportado de SIDERAL (2023-12-21-13:08:18)
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Brachypodium distachyon s. l. has been widely investigated across the world as a model plant for temperate cereals and biofuel grasses. However, this annual plant shows three cytotypes that have been recently recognized as three independent species, the diploids B. distachyon (2n = 10) and B. stacei (2n = 20) and their derived allotetraploid B. hybridum (2n = 30).
Methodology/Principal Findings
We propose a DNA barcoding approach that consists of a rapid, accurate and automatable species identification method using the standard DNA sequences of complementary plastid (trnLF) and nuclear (ITS, GI) loci. The highly homogenous but largely divergent B. distachyon and B. stacei diploids could be easily distinguished (100% identification success) using direct trnLF (2.4%), ITS (5.5%) or GI (3.8%) sequence divergence. By contrast, B. hybridum could only be unambiguously identified through the use of combined trnLF+ITS sequences (90% of identification success) or by cloned GI sequences (96.7%) that showed 5.4% (ITS) and 4% (GI) rate divergence between the two parental sequences found in the allopolyploid.
Conclusion/Significance
Our data provide an unbiased and effective barcode to differentiate these three closely-related species from one another. This procedure overcomes the taxonomic uncertainty generated from methods based on morphology or flow cytometry identifications that have resulted in some misclassifications of the model plant and its allies. Our study also demonstrates that the allotetraploid B. hybridum has resulted from bi-directional crosses of B. distachyon and B. stacei plants acting either as maternal or paternal parents.
Idioma: Inglés
DOI: 10.1371/journal.pone.0051058
Año: 2012
Publicado en: PLoS ONE 7, 12 (2012), e51058 [20 pp.]
ISSN: 1932-6203
Factor impacto JCR: 3.73 (2012)
Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 7 / 57 = 0.123 (2012) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/Bioflora
Financiación: info:eu-repo/grantAgreement/ES/MICINN/CGL2009-12955-C02-01
Tipo y forma: Article (Published version)
Área (Departamento): Área Botánica (Dpto. CC.Agrar.y Medio Natural)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. Exportado de SIDERAL (2023-12-21-13:08:18)
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Record created 2023-12-21, last modified 2023-12-21