A DNA Barcoding Method to Discriminate between the Model Plant Brachypodium distachyon and Its Close Relatives B. stacei and B. hybridum (Poaceae)
López-Alvarez, D. (Universidad de Zaragoza) ; López-Herranz, M. L. ; Betekhtin, A. ; Catalán, P. (Universidad de Zaragoza)
Resumen: Background
Brachypodium distachyon s. l. has been widely investigated across the world as a model plant for temperate cereals and biofuel grasses. However, this annual plant shows three cytotypes that have been recently recognized as three independent species, the diploids B. distachyon (2n = 10) and B. stacei (2n = 20) and their derived allotetraploid B. hybridum (2n = 30).
Methodology/Principal Findings
We propose a DNA barcoding approach that consists of a rapid, accurate and automatable species identification method using the standard DNA sequences of complementary plastid (trnLF) and nuclear (ITS, GI) loci. The highly homogenous but largely divergent B. distachyon and B. stacei diploids could be easily distinguished (100% identification success) using direct trnLF (2.4%), ITS (5.5%) or GI (3.8%) sequence divergence. By contrast, B. hybridum could only be unambiguously identified through the use of combined trnLF+ITS sequences (90% of identification success) or by cloned GI sequences (96.7%) that showed 5.4% (ITS) and 4% (GI) rate divergence between the two parental sequences found in the allopolyploid.
Conclusion/Significance
Our data provide an unbiased and effective barcode to differentiate these three closely-related species from one another. This procedure overcomes the taxonomic uncertainty generated from methods based on morphology or flow cytometry identifications that have resulted in some misclassifications of the model plant and its allies. Our study also demonstrates that the allotetraploid B. hybridum has resulted from bi-directional crosses of B. distachyon and B. stacei plants acting either as maternal or paternal parents.
Idioma: Inglés
DOI: 10.1371/journal.pone.0051058
Año: 2012
Publicado en: PLoS ONE 7, 12 (2012), e51058 [20 pp.]
ISSN: 1932-6203
Factor impacto JCR: 3.73 (2012)
Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 7 / 57 = 0.123 (2012) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/Bioflora
Financiación: info:eu-repo/grantAgreement/ES/MICINN/CGL2009-12955-C02-01
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Área Botánica (Dpto. CC.Agrar.y Medio Natural)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace.
Exportado de SIDERAL (2023-12-21-13:08:18)
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Brachypodium distachyon s. l. has been widely investigated across the world as a model plant for temperate cereals and biofuel grasses. However, this annual plant shows three cytotypes that have been recently recognized as three independent species, the diploids B. distachyon (2n = 10) and B. stacei (2n = 20) and their derived allotetraploid B. hybridum (2n = 30).
Methodology/Principal Findings
We propose a DNA barcoding approach that consists of a rapid, accurate and automatable species identification method using the standard DNA sequences of complementary plastid (trnLF) and nuclear (ITS, GI) loci. The highly homogenous but largely divergent B. distachyon and B. stacei diploids could be easily distinguished (100% identification success) using direct trnLF (2.4%), ITS (5.5%) or GI (3.8%) sequence divergence. By contrast, B. hybridum could only be unambiguously identified through the use of combined trnLF+ITS sequences (90% of identification success) or by cloned GI sequences (96.7%) that showed 5.4% (ITS) and 4% (GI) rate divergence between the two parental sequences found in the allopolyploid.
Conclusion/Significance
Our data provide an unbiased and effective barcode to differentiate these three closely-related species from one another. This procedure overcomes the taxonomic uncertainty generated from methods based on morphology or flow cytometry identifications that have resulted in some misclassifications of the model plant and its allies. Our study also demonstrates that the allotetraploid B. hybridum has resulted from bi-directional crosses of B. distachyon and B. stacei plants acting either as maternal or paternal parents.
Idioma: Inglés
DOI: 10.1371/journal.pone.0051058
Año: 2012
Publicado en: PLoS ONE 7, 12 (2012), e51058 [20 pp.]
ISSN: 1932-6203
Factor impacto JCR: 3.73 (2012)
Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 7 / 57 = 0.123 (2012) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/Bioflora
Financiación: info:eu-repo/grantAgreement/ES/MICINN/CGL2009-12955-C02-01
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Área Botánica (Dpto. CC.Agrar.y Medio Natural)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. Exportado de SIDERAL (2023-12-21-13:08:18)
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