Comparative study of hematopoietic stem and progenitor cells between sexes in mice under physiological conditions along time

Gasco, S. ; Rando, A. ; Zaragoza, P. (Universidad de Zaragoza) ; García-Redondo, A. ; Calvo, A.C. (Universidad de Zaragoza) ; Osta, R. (Universidad de Zaragoza)

Resumen: Hematopoietic stem and progenitor cells (HSPCs) are attractive targets in regenerative medicine, although the differences in their homeostatic maintenance between sexes along time are still under debate. We accurately monitored hematopoietic stem cells (HSCs), common lymphoid progenitors (CLPs), and common myeloid progenitors (CMPs) frequencies by flow cytometry, by performing serial peripheral blood extractions from male and female B6SJL wild-type mice and found no significant differences. Only modest differences were found in the gene expression profile of Slamf1 and Gata2. Our findings suggest that both sexes could be used indistinctly to perform descriptive studies in the murine hematopoietic system, especially for flow cytometry studies in peripheral blood. This would allow diminishing the number of animals needed for the experimental procedures. In addition, the use of serial extractions in the same animals drastically decreases the number of animals needed.

Idioma: Inglés

DOI: 10.1002/cbin.10865

Año: 2017

Publicado en: CELL BIOLOGY INTERNATIONAL 41, 12 (2017), 1399-1405
ISSN: 1065-6995

Factor impacto JCR: 1.936 (2017)

Categ. JCR: CELL BIOLOGY rank: 153 / 190 = 0.805 (2017) - Q4 - T3

Factor impacto SCIMAGO: 0.712 - Medicine (miscellaneous) (Q2) - Cell Biology (Q3)

Financiación: info:eu-repo/grantAgreement/ES/FIS/PI14-00947

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)