000130005 001__ 130005
000130005 005__ 20240319080955.0
000130005 0247_ $$2doi$$a10.1007/s00764-022-00171-7
000130005 0248_ $$2sideral$$a129911
000130005 037__ $$aART-2022-129911
000130005 041__ $$aeng
000130005 100__ $$aCebolla, Vicente L.
000130005 245__ $$aLipidomic studies based on high-performance thin-layer chromatography
000130005 260__ $$c2022
000130005 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130005 5203_ $$aThe last triennium has shown a significant contribution of high-performance thin-layer chromatography (HPTLC) to lipidomics. HPTLC separation in combination with radio- and/or ultraviolet-fluorescence (UV-FL) densitometry was the technique of choice for tracking the transport of phospholipids among different cellular compartments in a number of biological systems, e.g., Gram-negative bacteria, yeast membranes, endoplasmic reticulum/mitochondrial membrane interface, and also to monitor the lipid transfer activity of a protein. Likewise, a significant number of HPTLC methods were developed to determine variations in the content of different lipid classes and subclasses after using genetic knockouts of cells. Radiolabeled control cells and cells whose genes were deleted using genomic editing allowed to study by HPTLC the effect of the disruption of the related proteins on the corresponding biosynthetic pathways. As well, direct interface-based coupling of HPTLC to mass spectrometry (MS) using mostly electrospray ionization(ESI) and a variety of mass analyzers, has gained new momentum in this period. Obtaining a large amount of information online in a very short time from complex biological samples, and the structural identification of target and non-target, unknown lipids, make this technique a useful tool for lipidomics. Quantitative issues related to HPTLC-MS are also discussed in this work.
000130005 536__ $$9info:eu-repo/grantAgreement/ES/CSIC/2021-80E076$$9info:eu-repo/grantAgreement/ES/DGA/E25-20R$$9info:eu-repo/grantAgreement/ES/ISCIII-AES/PI21-00036
000130005 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000130005 590__ $$a1.6$$b2022
000130005 591__ $$aCHEMISTRY, ANALYTICAL$$b70 / 86 = 0.814$$c2022$$dQ4$$eT3
000130005 592__ $$a0.268$$b2022
000130005 593__ $$aAnalytical Chemistry$$c2022$$dQ3
000130005 593__ $$aClinical Biochemistry$$c2022$$dQ4
000130005 593__ $$aBiochemistry$$c2022$$dQ4
000130005 594__ $$a1.9$$b2022
000130005 655_4 $$ainfo:eu-repo/semantics/review$$vinfo:eu-repo/semantics/acceptedVersion
000130005 700__ $$aJarne, Carmen
000130005 700__ $$aMembrado, Luis
000130005 700__ $$0(orcid)0000-0002-5645-6723$$aEscuin, Jose M.
000130005 700__ $$0(orcid)0000-0002-4887-1652$$aVela, Jesús$$uUniversidad de Zaragoza
000130005 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000130005 773__ $$g35 (2022), 229-241$$pJPC, J. planar chromatogr. mod. TLC$$tJPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC$$x0933-4173
000130005 8564_ $$s2371190$$uhttps://zaguan.unizar.es/record/130005/files/texto_completo.pdf$$yPostprint
000130005 8564_ $$s2118675$$uhttps://zaguan.unizar.es/record/130005/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000130005 909CO $$ooai:zaguan.unizar.es:130005$$particulos$$pdriver
000130005 951__ $$a2024-03-18-13:34:37
000130005 980__ $$aARTICLE