Lipidomic studies based on high-performance thin-layer chromatography

Cebolla, Vicente L. ; Jarne, Carmen ; Membrado, Luis ; Escuin, Jose M. ; Vela, Jesús (Universidad de Zaragoza)

Resumen: The last triennium has shown a significant contribution of high-performance thin-layer chromatography (HPTLC) to lipidomics. HPTLC separation in combination with radio- and/or ultraviolet-fluorescence (UV-FL) densitometry was the technique of choice for tracking the transport of phospholipids among different cellular compartments in a number of biological systems, e.g., Gram-negative bacteria, yeast membranes, endoplasmic reticulum/mitochondrial membrane interface, and also to monitor the lipid transfer activity of a protein. Likewise, a significant number of HPTLC methods were developed to determine variations in the content of different lipid classes and subclasses after using genetic knockouts of cells. Radiolabeled control cells and cells whose genes were deleted using genomic editing allowed to study by HPTLC the effect of the disruption of the related proteins on the corresponding biosynthetic pathways. As well, direct interface-based coupling of HPTLC to mass spectrometry (MS) using mostly electrospray ionization(ESI) and a variety of mass analyzers, has gained new momentum in this period. Obtaining a large amount of information online in a very short time from complex biological samples, and the structural identification of target and non-target, unknown lipids, make this technique a useful tool for lipidomics. Quantitative issues related to HPTLC-MS are also discussed in this work.

Idioma: Inglés

DOI: 10.1007/s00764-022-00171-7

Año: 2022

Publicado en: JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC 35 (2022), 229-241
ISSN: 0933-4173

Factor impacto JCR: 1.6 (2022)

Categ. JCR: CHEMISTRY, ANALYTICAL rank: 70 / 86 = 0.814 (2022) - Q4 - T3

Factor impacto CITESCORE: 1.9 - Chemistry (Q3) - Biochemistry, Genetics and Molecular Biology (Q4)

Factor impacto SCIMAGO: 0.268 - Analytical Chemistry (Q3) - Clinical Biochemistry (Q4) - Biochemistry (Q4)

Financiación: info:eu-repo/grantAgreement/ES/CSIC/2021-80E076
Financiación: info:eu-repo/grantAgreement/ES/DGA/E25-20R
Financiación: info:eu-repo/grantAgreement/ES/ISCIII-AES/PI21-00036

Tipo y forma: Revisión (PostPrint)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)