000130243 001__ 130243
000130243 005__ 20240123131022.0
000130243 0247_ $$2doi$$a10.1016/j.transproceed.2012.05.006
000130243 0248_ $$2sideral$$a78271
000130243 037__ $$aART-2012-78271
000130243 041__ $$aeng
000130243 100__ $$aGonzalo, M. A.
000130243 245__ $$aAnalysis of tissue bioimpedance as a measurement of liver steatosis: Experimental model in large animals
000130243 260__ $$c2012
000130243 5203_ $$aBackground
Electrical bioimpedance (BI) has been used to indirectly measure steatosis. This method has not yet been established in the clinics thus experimental studies are needed in big animals. We assessed BI to measure liver steatosis in porcine animals.
Methods
Twelve large-white × Landrace pigs weighing 35 kg were allocated to a study (n = 9) and a control group (n = 3). A special diet was used to promote steatosis among the study group: methionine deficient and choline-restricted diet that contains supplements of cholesterol, collate and excess of saturated fat. Control group animals were fed a normal diet. A new tetrapolar electrode model was used for BI measurement, which were performed during open laparotomy by inserting a probe into one of the lobes. Measurements were done in the third and fourth segments of the pig liver, placing the probe either on the surface or inserted into the parenchyma of the liver. Open biopsies were obtained at the end of the measurements. Histological samples were processed and stained with hematoxylin-eosin to estimate macrosteatosis. Pearson correlation coefficient between BI and percentage steatosis were calculated at different frequencies.
Results
After 4 months of the special diet all the animals in the study group developed steatosis (90% to 20%), whereas none of the control group was affected. Pearson correlation coefficients between BI and percentage of steatosis were significant (0.877–0.878) with the best correlations obtained with a probe placed on the fourth segment of the liver surface and the best frequency to perform the measurements being 50 and 75 kHz.
Conclusions
BI is an accurate, fast method for steatosis measurements, that is easier and cheaper than either open or needle biopsy.
000130243 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000130243 590__ $$a0.952$$b2012
000130243 591__ $$aSURGERY$$b130 / 198 = 0.657$$c2012$$dQ3$$eT2
000130243 591__ $$aTRANSPLANTATION$$b22 / 26 = 0.846$$c2012$$dQ4$$eT3
000130243 591__ $$aIMMUNOLOGY$$b124 / 136 = 0.912$$c2012$$dQ4$$eT3
000130243 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000130243 700__ $$0(orcid)0000-0002-8100-5596$$aMartínez-Beamonte, R.
000130243 700__ $$aPalacios,P.
000130243 700__ $$aMarín, J.
000130243 700__ $$0(orcid)0000-0001-7453-2470$$aCastiella, T.$$uUniversidad de Zaragoza
000130243 700__ $$0(orcid)0000-0002-5841-0462$$aSurra, J.$$uUniversidad de Zaragoza
000130243 700__ $$aBurdío, F.
000130243 700__ $$0(orcid)0000-0002-8102-5730$$aSousa, R.$$uUniversidad de Zaragoza
000130243 700__ $$0(orcid)0000-0002-4437-2581$$aGüemes, A.$$uUniversidad de Zaragoza
000130243 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, J.$$uUniversidad de Zaragoza
000130243 700__ $$aGarcía-Gil, A.
000130243 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000130243 7102_ $$11004$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Cirugía
000130243 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000130243 7102_ $$11000$$2020$$aUniversidad de Zaragoza$$bDpto. Anat.Pat.Med.Leg.For.To.$$cArea Anatomía Patológica
000130243 773__ $$g44, 6 (2012), 1579-1583$$pTransplant. proc.$$tTransplantation Proceedings$$x0041-1345
000130243 8564_ $$s1747042$$uhttps://zaguan.unizar.es/record/130243/files/texto_completo.pdf$$yPostprint
000130243 8564_ $$s2498726$$uhttps://zaguan.unizar.es/record/130243/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000130243 909CO $$ooai:zaguan.unizar.es:130243$$particulos$$pdriver
000130243 951__ $$a2024-01-23-12:42:55
000130243 980__ $$aARTICLE