Analysis of tissue bioimpedance as a measurement of liver steatosis: Experimental model in large animals
Gonzalo, M. A. ; Martínez-Beamonte, R. ; Palacios,P. ; Marín, J. ; Castiella, T. (Universidad de Zaragoza) ; Surra, J. (Universidad de Zaragoza) ; Burdío, F. ; Sousa, R. (Universidad de Zaragoza) ; Güemes, A. (Universidad de Zaragoza) ; Osada, J. (Universidad de Zaragoza) ; García-Gil, A.
Resumen: Background
Electrical bioimpedance (BI) has been used to indirectly measure steatosis. This method has not yet been established in the clinics thus experimental studies are needed in big animals. We assessed BI to measure liver steatosis in porcine animals.
Methods
Twelve large-white × Landrace pigs weighing 35 kg were allocated to a study (n = 9) and a control group (n = 3). A special diet was used to promote steatosis among the study group: methionine deficient and choline-restricted diet that contains supplements of cholesterol, collate and excess of saturated fat. Control group animals were fed a normal diet. A new tetrapolar electrode model was used for BI measurement, which were performed during open laparotomy by inserting a probe into one of the lobes. Measurements were done in the third and fourth segments of the pig liver, placing the probe either on the surface or inserted into the parenchyma of the liver. Open biopsies were obtained at the end of the measurements. Histological samples were processed and stained with hematoxylin-eosin to estimate macrosteatosis. Pearson correlation coefficient between BI and percentage steatosis were calculated at different frequencies.
Results
After 4 months of the special diet all the animals in the study group developed steatosis (90% to 20%), whereas none of the control group was affected. Pearson correlation coefficients between BI and percentage of steatosis were significant (0.877–0.878) with the best correlations obtained with a probe placed on the fourth segment of the liver surface and the best frequency to perform the measurements being 50 and 75 kHz.
Conclusions
BI is an accurate, fast method for steatosis measurements, that is easier and cheaper than either open or needle biopsy.
Idioma: Inglés
DOI: 10.1016/j.transproceed.2012.05.006
Año: 2012
Publicado en: Transplantation Proceedings 44, 6 (2012), 1579-1583
ISSN: 0041-1345
Factor impacto JCR: 0.952 (2012)
Categ. JCR: SURGERY rank: 130 / 198 = 0.657 (2012) - Q3 - T2
Categ. JCR: TRANSPLANTATION rank: 22 / 26 = 0.846 (2012) - Q4 - T3
Categ. JCR: IMMUNOLOGY rank: 124 / 136 = 0.912 (2012) - Q4 - T3
Tipo y forma: Artículo (PostPrint)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)
Área (Departamento): Área Cirugía (Dpto. Cirugía,Ginecol.Obstetr.)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Area Anatomía Patológica (Dpto. Anat.Pat.Med.Leg.For.To.)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. No puede utilizar el material para una finalidad comercial. Si remezcla, transforma o crea a partir del material, no puede difundir el material modificado.
Exportado de SIDERAL (2024-01-23-12:42:55)
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Electrical bioimpedance (BI) has been used to indirectly measure steatosis. This method has not yet been established in the clinics thus experimental studies are needed in big animals. We assessed BI to measure liver steatosis in porcine animals.
Methods
Twelve large-white × Landrace pigs weighing 35 kg were allocated to a study (n = 9) and a control group (n = 3). A special diet was used to promote steatosis among the study group: methionine deficient and choline-restricted diet that contains supplements of cholesterol, collate and excess of saturated fat. Control group animals were fed a normal diet. A new tetrapolar electrode model was used for BI measurement, which were performed during open laparotomy by inserting a probe into one of the lobes. Measurements were done in the third and fourth segments of the pig liver, placing the probe either on the surface or inserted into the parenchyma of the liver. Open biopsies were obtained at the end of the measurements. Histological samples were processed and stained with hematoxylin-eosin to estimate macrosteatosis. Pearson correlation coefficient between BI and percentage steatosis were calculated at different frequencies.
Results
After 4 months of the special diet all the animals in the study group developed steatosis (90% to 20%), whereas none of the control group was affected. Pearson correlation coefficients between BI and percentage of steatosis were significant (0.877–0.878) with the best correlations obtained with a probe placed on the fourth segment of the liver surface and the best frequency to perform the measurements being 50 and 75 kHz.
Conclusions
BI is an accurate, fast method for steatosis measurements, that is easier and cheaper than either open or needle biopsy.
Idioma: Inglés
DOI: 10.1016/j.transproceed.2012.05.006
Año: 2012
Publicado en: Transplantation Proceedings 44, 6 (2012), 1579-1583
ISSN: 0041-1345
Factor impacto JCR: 0.952 (2012)
Categ. JCR: SURGERY rank: 130 / 198 = 0.657 (2012) - Q3 - T2
Categ. JCR: TRANSPLANTATION rank: 22 / 26 = 0.846 (2012) - Q4 - T3
Categ. JCR: IMMUNOLOGY rank: 124 / 136 = 0.912 (2012) - Q4 - T3
Tipo y forma: Artículo (PostPrint)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)
Área (Departamento): Área Cirugía (Dpto. Cirugía,Ginecol.Obstetr.)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Area Anatomía Patológica (Dpto. Anat.Pat.Med.Leg.For.To.)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. No puede utilizar el material para una finalidad comercial. Si remezcla, transforma o crea a partir del material, no puede difundir el material modificado.Exportado de SIDERAL (2024-01-23-12:42:55)
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Este artículo se encuentra en las siguientes colecciones:
Artículos > Artículos por área > Bioquímica y Biología Molecular
Artículos > Artículos por área > Anatomía Patológica
Artículos > Artículos por área > Producción Animal
Artículos > Artículos por área > Cirugía
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