Higher platelet cytochrome oxidase specific activity in surviving than in non-surviving septic patients

Lorente, L. ; Martín, M. M. ; López-Gallardo, E. (Universidad de Zaragoza) ; Iceta, R. ; Blanquer, J. ; Solé-Violán, J. ; Labarta, L. ; Díaz, C. ; Jiménez, A. (Universidad de Zaragoza) ; Montoya, J. ; Ruiz-Pesini, E. (Universidad de Zaragoza)

Resumen: Introduction: In a previous study with 96 septic patients, we found that circulating platelets in 6-months surviving septic patients showed higher activity and quantity of cytochrome c oxidase (COX) normalized by citrate synthase (CS) activity at moment of severe sepsis diagnosis than non-surviving septic patients. The objective of this study was to estimate whether COX specific activity during the first week predicts 1-month sepsis survival in a larger cohort of patients.Methods: Using a prospective, multicenter, observational study carried out in six Spanish intensive care units with 198 severe septic patients, we determined COX activity per proteins (COXact/Prot) in circulating platelets at day 1, 4 and 8 of the severe sepsis diagnosis. Endpoints were 1-month and 6-months mortality.Results: Survivor patients (n = 130) showed higher COXact/Prot (P 0.30 mOD/min/mg at day 1 (P = 0.002), 4 (P = 0.006) and 8 (P = 0.02) was associated independently with 1-month mortality. Area under the curve of COXact/Prot at day 1, 4 and 8 to predict 30-day survival were 0.70 (95% CI = 0.63-0.76; P < 0.001), 0.71 (95% CI = 0.64-0.77; P < 0.001) and 0.71 (95% CI = 0.64-0.78; P < 0.001), respectively.Conclusions: The new findings of our study, to our knowledge the largest series reporting data about mitochondrial function during follow-up in septic patients, were that septic patients that survive 1-month have a higher platelet cytochrome oxidase activity at moment of sepsis diagnosis and during the first week than non-survivors, and that platelet cytochrome oxidase activity at moment of sepsis diagnosis and during the first week could be used as biomarker to predict the clinical outcome in septic patients.

Idioma: Inglés

DOI: 10.1186/cc13956

Año: 2014

Publicado en: Critical care 18, 3 (2014), R136 [17 pp]
ISSN: 1364-8535

Factor impacto JCR: 4.476 (2014)

Categ. JCR: CRITICAL CARE MEDICINE rank: 5 / 27 = 0.185 (2014) - Q1 - T1

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)